诱导多能干细胞在心血管疾病中的应用:效率及安全性  

Application of induced pluripotent stem cells in cardiovascular diseases: efficiency and safety

作　　者：曾建银 韩燊[1] 李亚雄[1] 刘斌[1] 张雅永[1] 蒋立虹[1] Zeng Jianyin;Han Shen;Li Yaxiong;Liu Bin;Zhang Yayong;Jiang Lihong(Department of Cardiac Vascular Surgery,Yan’an Hospital,Kunming Medical University,Yunnan Institute of Cardiovascular Surgery,Yunnan Key Laboratory of Cardiovascular Diseases,Kunming 650051,Yunnan Province,China)

机构地区：[1]昆明医科大学附属延安医院心脏大血管外科昆明市延安医院心脏大血管外科云南省心血管外科研究所云南省心血管疾病重点实验室云南省第一人民医院,云南省昆明市650051

出　　处：《中国组织工程研究》2019年第9期1448-1454,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家临床重点专科建设项目;云南省心血管外科治疗技术创新团队(2015HC015);项目负责人:李亚雄;云南省卫生科技计划项目;诱导心血管疾病患者iPSC向心肌细胞分化及其功能的研究(2017NS328);项目负责人:张雅永;云南省科技创新人才计划;昆明市延安医院心脏大血管外科关键诊疗技术研究省创新团队(2018HC027);项目负责人:李亚雄~~

摘　　要：背景:诱导多能干细胞是具有与胚胎干细胞相似特征的一类重编程细胞,能够定向分化成与患者特异性疾病相关表型的细胞,并且其临床应用避免了伦理道德问题。目的:综述诱导多能干细胞在心肌再生与修复、心血管疾病模型、药物研发与筛选、药物心脏毒性检测等应用中的研究进展。方法:检索PubMed数据库2006至2018年期间的相关文章,检索词为"induction of pluripotent stem cells,myocardial infarction,arrhythmia,cardiovascular disease,heart failure,heart transplantation,disease models,drugtoxicity",同时检索中国知网数据库2013至2018年期间的相关文章,检索词为"诱导多能干细胞,心血管疾病,心肌梗死,心律失常,心力衰竭,疾病模型,药物毒性"。对资料进行逐一初审,并查看每篇文献后的引文。结果与结论:诱导多能干细胞在心肌再生与修复、心血管疾病模型的建立、新药研发与筛选、药物心脏毒性检测等方面具有巨大的潜在应用价值,应用前景广阔,但是大部分研究尚处于实验研究阶段;另外其诱导效率低、存在致瘤性的安全问题将限制诱导多能干细胞的临床应用。BACKGROUND:Induced pluripotent stem cells are a type of reprogrammed cells with similar characteristics to embryonic stem cells,which are capable of differentiating into phenotypes associated with patient specific diseases.Moreover,their clinical application avoids ethical issues.OBJECTIVE:To review the research progress of induced pluripotent stem cells in myocardial regeneration and repair,cardiovascular disease models,drug development and screening,and drug toxicity testing.METHODS:PubMed(2006-2018)and CNKI(2013-2018)databases were retrieved for relevant articles using the keywords of“induction of pluripotent stem cells;myocardial infarction;arrhythmia;cardiovascular disease;heart failure;heart transplantation;disease model;drug toxicity”in English and Chinese,respectively.The data were reviewed one by one,and the citations involved in the literatures were also reviewed.RESULTS AND CONCLUSION:Induced pluripotent stem cells have great potential value in myocardial regeneration and repair,establishment of cardiovascular disease models,new drug development and screening,and drug toxicity detection.The application prospect of the cells is broad,but most of the research is still in the experimental stage.In addition,safety problems,such as low induction efficiency and tumorigenicity,will limit the clinical application of induced pluripotent stem cells.

关 键 词：诱导多能干细胞 心血管疾病 心肌梗死 心律失常 心力衰竭 药物毒性 诱导多功能干细胞 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学]