外源尿黑酸对拟南芥幼苗花青素生物合成的影响  

Effect of exogenous homogentisate on anthocyanin biosynthesis in Arabidopsis thaliana seedlings

作　　者：汤睿 雷雨婷 任春梅[1,2] TANG Rui;LEI Yuting;REN Chunmei(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,Hunan 410128,China;Crop Gene Engineering Key Laboratory of Hunan Province,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]作物基因工程湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2019年第1期37-41,共5页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家"973"计划前期研究专项(2014CB160308)

摘　　要：以拟南芥Col–0(CK)和酪氨酸降解最后一步缺陷突变体sscd1为试验材料,采用外源尿黑酸处理幼苗,研究尿黑酸对花青素生物合成的影响。结果显示:外源尿黑酸能诱导拟南芥幼苗花青素的积累,而且在sscd1突变体中花青素的积累效果更显著;荧光定量PCR分析发现,尿黑酸处理能促进花青素生物合成基因(包括花青素生物合成的下游基因DFR、LDOX、UF3GT和上游基因PAL、CHI、CHS)的表达,且在sscd1突变体中,这些基因表达上调的幅度更大。以上结果表明,尿黑酸处理能激活花青素生物合成途径,从而促进花青素的积累;酪氨酸降解最后一步缺陷能促进尿黑酸诱导花青素的生物合成。In this study,we used Arabidopsis model plant of wild-type and tyrosine pathway-deficient mutant sscd1 in the last step as the experimental materials,analyzed the effect of homogentisate on the synthesis of anthocyanin with the treatment of exogenous homogentisate.The results showed that the exogenous homogentisate could induce anthocyanin accumulation,the accumulation of mutant sscd1 was more significant.Furthermore,the expression of anthocyanin synthesis was analyzed by qRT-PCR.The results showed that homogentisate could promote the expression of upstream genes PAL,CHI,CHS and downstream genes DFR,LDOX,UF3GT of anthocyanin biosynthesis,similarly,the gene expression in sscd1 mutants was up-regulated more.The above results indicated that:the treatment of homogentisate activated the anthocyanin biosynthetic pathway and lead to anthocyanin accumulation.Defects in the final step of the tyrosine degradation pathway could increase the biosynthesis ofanthocyanin which induced by homogentisate.

关 键 词：拟南芥 sscd1突变体 花青素 尿黑酸 酪氨酸降解途径 

分 类 号：Q786[生物学—分子生物学]