中药谱效学研究过程中差异样品的构建及应用  

A novel approach to prepare the difference samples of traditional Chinese herbal formulae and its application

作　　者：刘宏[1] 苏薇薇[1] 彭维[1] 姚宏亮[1] LIU Hong;SU Weiwei;PENG Wei;YAO Hongliang(School of Life Sciences∥Sun Yat-sen University, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post-Marketed TCM, Guangzhou 510275, China)

机构地区：[1]中山大学生命科学学院∥广东省中药上市后质量与药效再评价工程技术研究中心,广东广州510275

出　　处：《中山大学学报（自然科学版）》2019年第1期126-130,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省名优中成药二次开发项目(2017-No.19);中山大学成果转化项目-中药上市后质量与药效再评价核心关键技术成果的转化(33000-18825004)

摘　　要：提出中药谱效学研究过程中差异样品的构建方法:将每味药材看作试验因素,根据原中药组方比例确定各味药材的投料量变化范围,并在该范围内确定合适的水平数;采用均匀设计法,根据因素数、水平数选择合适的均匀设计表,设计几组不同的药材配比;按照标准工艺流程制备差异样品,并用指纹图谱进行监控,采用聚类分析法验证不同差异样品之间的差异性。该方法不同于以往的化学成分逐一分离、再组合的方式,而是从整体上对各味药材用量进行调整,具有实现难度小、适用性高的优点,符合中药的混合性、整体性、统一性特点。A novel approach to prepare the difference samples of traditional Chinese herbal formulae (CHF) was established, the strategy of the approach are: regarding the herb as the test factors, defining the suitable test levels based on the original herb proportion, designing the different herb proportion using Uniform Design method, preparing the different samples according to standard process, analyzing the HPLC chromatography of the difference samples using Cluster Analysis. Different from traditional pattern of separating and combining the component one by one, the proposed approach re-adjusts the herbs as a whole with the advantages of low difficulty and high applicability, conforming to the CHF characters of mixture, integrity, and unity.

关 键 词：复方中药 差异样品 复方血栓通胶囊 

分 类 号：R96[医药卫生—药理学]