干旱胁迫对何首乌生长和叶绿素荧光参数的影响  

Effects of Drought Stress on Growth and Chlorophyll Fluorescence Parameters of Polygonum multiflorum

作　　者：刘筱 王锐洁 杨淑君 姬拉拉 王健健[1] LIU Xiao;WANG Ruijie;YANG Shujun;JI Lala;WANG Jianjian(College of Life Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025

出　　处：《河南农业科学》2019年第1期50-56,共7页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31760155);贵州省科技厅自然科学基金项目[2016(1033)];贵州省农业领域科技成果转化重点项目(黔科合成转字[2015]5021号)

摘　　要：为明确干旱胁迫对贵州何首乌生长和叶绿素荧光参数的影响,设置4个不同干旱处理模拟不同降水情景(CK:1 d 1次灌水,W1处理:3 d 1次灌水,W_2处理:5 d 1次灌水,W_3处理:7 d 1次灌水),测定干旱胁迫下何首乌的生长指标和叶绿素荧光参数。结果表明:随干旱胁迫加剧,何首乌总叶面积、叶长、叶片数、叶宽、叶厚、株高、分枝数、根长显著降低,降低程度表现为W_3> W_2>W_1,须根和块根干质量先升高后降低,叶、茎干质量均呈降低趋势且在W_3处理下达最小值,根冠比和根生物量比显著增加,较CK分别增加4. 03倍和97. 14%;随干旱胁迫加剧,何首乌各组织含水量呈先降低后增加趋势;随干旱胁迫加剧,叶绿素含量、Fv/Fm、ETR和qP值呈先升高后降低的趋势,NPQ呈降低趋势,叶绿素荧光参数均在W_3处理下降至最小值,可见,何首乌光系统Ⅱ在W_3处理下遭受不可恢复的损伤。综上,W_3处理下何首乌生长受到较大的抑制,叶绿素荧光参数最低,何首乌幼苗的自我修复能力受到严重损害,超出了何首乌幼苗的耐性阈值。In order to clarify the effects of drought stress on the growth and chlorophyll fluorescence parameters of Polygonum multiflorum grown in Guizhou province,four different drought treatments(CK:irrigate once per day,W1:irrigate once every 3 days,W2:irrigate once every 5 days,W3:irrigate once every 7 days)were set up to simulate different rainfall conditions,and growth and chlorophyll fluorescence parameters of Polygonum multiflorum under drought stress were recorded.The results showed that:with the increase of drought stress,total leaf area,leaf length,leaf number,leaf width,leaf thickness,plant height,branch number and root length of Polygonum multiflorum were significantly decreased,and the decreasing degree was W3﹥W2﹥W1.The dry weight of fine roots and thick roots increased firstly and then decreased,while dry weight of leaves and stem showed a downward trend and W3 treatment reached the lowest.Although the root-shoot ratio and root biomass ratio increased significantly 4.03 times and 97.14%than CK respectively;with the increase of drought stress,tissue moisture content of Polygonum multiflorum decreased firstly and then increased,the chlorophyll content,Fv/Fm,ETR and qP increased firstly and then decreased,while NPQ decreased,and the chlorophyll fluorescence parameters reached the minimum under W3 treatment,which meant light systemⅡof Polygonum multiflorum suffered irreversible damage.In conclusion,under W3 treatment,the growth of Polygonum multiflorum was inhibited mostly,the chlorophyll fluorescence parameters were the lowest,and the self-repairing ability of Polygonum multiflorum seedlings suffered serious damages,which exceeded its tolerance threshold.

关 键 词：何首乌 干旱胁迫 生长 干物质量 荧光参数 

分 类 号：S567[农业科学—中草药栽培]