双参通脉颗粒对急性心肌缺血再灌注后的大鼠心肌细胞保护作用的影响  

Effect of Shuangshen Tongmai Granules on Myocardial Cell Protection in Rats after Acute Myocardial Ischemia-reperfusion

作　　者：吴迪[1] 刘彤[2] 杨莺[2] Wu Di;Liu Tong;Yang Ying(Liaoning University of Traditional Chinese Medicine,Liaoning,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中国中医急症》2019年第1期17-20,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：辽宁省自然科学基金项目(2015020392)

摘　　要：目的观察双参通脉颗粒对急性心肌缺血再灌注大鼠心肌细胞及心肌组织病理结构变化的影响。方法 40只SD大鼠随机分为4组:空白对照组、模型组、中药组、西药组。对模型组、中药组、西药组动物进行左冠状动脉前降支下2 mm处结扎建立急性心肌缺血再灌注模型,空白对照组只进行开胸手术不结扎,模型组及空白对照组用生理盐水灌胃,中药组予双参通脉颗粒,西药组予合心爽,用药治疗2周。治疗2周后,应用免疫组织化学技术检测大鼠心肌组织中血管细胞黏附分子(VCAM-1)及肿瘤坏死因子-α(TNF-α)的表达情况,并与空白对照组及模型组做比较。结果空白对照组中VCAM-1蛋白及TNF-α在心肌组织中表达程度低。模型组可观察到VCAM-1蛋白及TNF-α在心肌组织中的高表达。中药组的心肌组织VCAM-1及TNF-α的表达程度较模型组明显下降,介于空白组与西药组之间。西药组的心肌组织中VCAM-1及TNF-α的表达程度较模型组明显下降,介于中药组及模型组之间。且中药组与模型组相比较,差异具有统计学意义(P <0.05)。结论双参通脉颗粒可明显降低急性心肌缺血后再灌注的心肌组织损伤的程度,对心肌缺血后再灌注心肌的治疗有重要指导意义。Objective: To observe the effect of Shuangshen Tongmai Granules on the pathological changes of myocardial cells and myocardial tissue in rats with acute myocardial ischemia-reperfusion. Methods: 40 SD rats were randomly divided into four groups: the blank group,the model group,Shuangshen Tongmai Granules group(TCM group) and positive control group(WM group). The model group,TCM group and WM group were subjected to an acute myocardial ischemia-reperfusion model. The blank control group was only subjected to thoracotomy without ligation. The model group and the blank group were intragastrically administered with saline. Shuangshen Tongmai Granules and Hexinshuang were given routinely in TCM group and WM group respectively after operation. After 2 weeks of treatment,the expression of VCAM-1 and TNF-alpha in myocardium of rats was detected by immunohistochemical technique,and compared with the blank group and the model group. Results: The expressions of VCAM-1 protein and TNF-α were in low the blank control group,and high in the model group.Those of TCM group,as well as WM group,were significantly decreased compared with the model group,and were between the blank group and WM group. Those of WM group were between the model group and TCM group. The difference between TCM group and the model group was statistically significant(P < 0.05). Conclusion: Shuangshen Tongmai Granules can significantly reduce the degree of myocardial injury after acute myocardial ischemiareperfusion,with an important guiding significance for its treatment.

关 键 词：双参通脉颗粒 急性心肌缺血再灌注 病理 VCAM-1 TNF-α 

分 类 号：R285.5[医药卫生—中药学]