微滤法分离人血中脱落上皮细胞  

Microfiltration to Isolate the Exfoliated Epithelial Cells in Human Blood for STR Test

作　　者：朱典[1] 高珊[1] 张颖[1] 张广峰[1] 聂昊[1] 刘开会[1] ZHU Dian;GAO Shan;ZHANG Ying;ZHANG Guangfeng;NIE Hao;LIU Kaihui(Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2019年第1期79-82,共4页Forensic Science and Technology

基　　金：基本科研业务费专项资金发展支撑项目(No.2016JB022)

摘　　要：目的建立基于微滤技术分离检验人血中脱落上皮细胞的新方法。方法选用微孔滤膜并构建细胞分离过滤平台,优化过滤参数;制备15组混合细胞悬液,每组包含2个等比例混合的样本, 1个进行常规DNA检验,另1个经过滤平台过滤,取滤膜作DNA检验,对比两者的STR分型结果,评判过滤平台的细胞分离效果。结果当流量为250mL/h、滤膜孔径30μm时,白细胞滤除率可达90%以上,而脱落上皮细胞截留率不低于70%;对15组混合细胞悬液经分离检验, 14组获得预期结果。结论该方法可用于人血中脱落上皮细胞的分离检验,能够改善此类检材中目的细胞的STR分型结果,为刑事案件中血迹浸染的烟蒂等混合生物检材的细胞分离检验提供了一种新思路。Objective To establish a new method for exfoliated epithelial cells to isolate from the contaminated human blood based on microfiltration technology.Methods A filtering platform was built from the selected microfiltration membrane for cell isolation,with optimization of the items of pore size and flowing rate.15 groups of equally-pair-mixed individualdifferent cell suspensions were prepared,with each group containing two identical suspensions,one of which was directly treated with the routine standard DNA extraction/test and the other firstly with the filtering platform and then undergone the DNA release and harvest so that the STR typing was carried out and compared with the corresponding standard one.Results When flowing rate is at 250mL/h and membrane-pore of 30μm,the removal of white blood cells reaches up to 90%,with more than 70%of the exfoliated epithelial cells being retained.14 out of the 15 groups of the suspensions meet the desired requirements.Conclusions The method is applicable to the isolation of exfoliated epithelial cells in human blood,helpful to optimize the STR typing of the target cells in mixed samples,thus providing a new approach in dealing with mixed biological samples,e.g.,the blood-stained cigarette butts.

关 键 词：微孔滤膜 细胞分离 DNA检验 混合生物检材 

分 类 号：DF795.2[医药卫生—法医学]