力生长因子在兔膝关节软骨细胞增殖和迁移中的作用  

Role of mechano growth factor in the proliferation and migration of rabbit knee articular chondrocytes

作　　者：赵会[1] 周君琳[1] 杨铁军[1] 李一汉[1] 郭蒙 Zhao Hui;Zhou Junlin;Yang Tiejun;Li Yihan;Guo Meng(Department of Orthopedics,Beijing Chao-Yang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院骨科,北京市100020

出　　处：《中国组织工程研究》2019年第11期1674-1679,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81202124);项目负责人:赵会;北京市医院管理局"青苗"计划(QML20160302);项目负责人:赵会~~

摘　　要：背景:力生长因子在多种组织中表达,能够促进多种组织细胞的增殖和迁移。目的:观察力生长因子对原代培养兔软骨细胞增殖和迁移的影响,以及内在机制探讨。方法:取北京市维通利华实验动物技术有限公司提供的新西兰大白兔的膝关节软骨,剪碎后,采用酶消化法原代分离培养软骨细胞,取处于对数生长期第3代细胞随机分5组,分别以0,1,2,4,8μg/L力生长因子进行干预24,48,72 h。结果和结论:不同质量浓度的力生长因子均能促进兔软骨细胞的增殖和迁移,且效应具有剂量依赖性,当质量浓度为4μg/L时力生长因子促增殖和迁移的效应最大。且随着力生长因子质量浓度逐渐增加,可明显增强兔软骨细胞中转化生长因子β1的表达,刺激Smad2/3的磷酸化。提示力生长因子对原代培养兔软骨细胞具有促增殖和迁移的作用,且具有剂量依赖性,可能是通过激活转化生长因子β1/Smad信号通路得以实现的。BACKGROUND:Mechano growth factor exists in a wide variety of tissues,and it can enhance the proliferation and migration of cells in different tissues.OBJECTIVE:To investigate the effect of mechano growth factor on the proliferation and migration of primary rabbit knee articular chondrocytes,and to explore the underlying mechanism.METHODS:Knee cartilage was shredded after being harvested from New Zealand white rabbits(provided by Beijing Vital River Laboratory Animal Technology Co.,Ltd.),and then chondrocytes were isolated and cultured by enzyme digestion.Passage 3 cells in logarithmic growth phase were harvested and divided into five groups,and then intervened by 0,1,2,4 and 8μg/L mechano growth factor for 24,48 and 72 hours,respectively.RESULTS AND CONCLUSION:Mechano growth factor could promote the proliferation and migration of rabbit knee articular chondrocytes in a concentration-dependent manner,and mechano growth factor at 4μg/L exhibited the strongest efficacy.Mechano growth factor could increase the expression of transforming growth factorβ1 and phosphorylation of Smad2/3 with concentration increasing.In summary,mechano growth factor can promote the proliferation and migration of rabbit knee articular chondrocytes dose-dependently,which may be via the transforming growth factorβ1/Smad signaling pathway.

关 键 词：软骨细胞 力生长因子 增殖 迁移 转化生长因子β1 成骨细胞 韧带细胞 膝关节软骨 细胞因子类 SMAD蛋白质类 转化生长因子 组织工程 

分 类 号：R456[医药卫生—治疗学] R363[医药卫生—临床医学]