山茱萸总甙干预骨质疏松模型大鼠骨代谢:TRPV6、TRPV5通路的变化  

Cornus officinalis glycosides intervening bone metabolism in rat models of osteoporosis:changes of TRPV6 and TRPV5 pathways

作　　者：李绍烁 赵京涛 何昌强 孙汉桥 阮冠龙 Li Shaoshuo;Zhao Jingtao;He Changqiang;Sun Hanqiao;Ruan Guanlong(Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Shenzhen Luohu District Hospital of Traditional Chinese Medicine,Shenzhen 518021,Guangdong Province,China;Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,Hubei Province,China)

机构地区：[1]广州中医药大学,广东省广州市510405 [2]广州中医药大学第一附属医院,广东省广州市510405 [3]深圳市罗湖区中医院,广东省深圳市518021 [4]湖北省中医院,湖北省武汉市430061

出　　处：《中国组织工程研究》2019年第11期1749-1754,共6页Chinese Journal of Tissue Engineering Research

基　　金：广东省自然科学基金(S2013010012540);项目负责人:赵京涛~~

摘　　要：背景:钙离子通道的调控直接影响骨的代谢,但骨代谢与TRPV6、TRPV5通路的联系及相互作用目前尚未明确。研究认为山茱萸具有防治骨质疏松作用。目的:探讨山茱萸总甙调节TRPV6、TRPV5通路表达防治骨质疏松症的机制。方法:健康6月龄雌性SD大鼠50只,体质量(350±15) g,由广州中医药大学实验动物中心提供。随机将大鼠分为5组,即正常对照组,模型组,山茱萸总甙干预低剂量组、中剂量组及高剂量组。除正常对照组外,其余各组大鼠行双侧卵巢切除术建立骨质疏松模型。造模后山茱萸总甙干预各组依剂量每天予药物灌胃,正常对照组及模型组予等量生理盐水灌胃,共12周。实验结束时,双能X射线骨密度仪扫描各组大鼠获取骨密度,取右侧股骨组织RT-PCR检测TRPV6、TRPV5 RNA基因表达情况。结果与结论:(1)山茱萸干预各组大鼠骨密度均较模型组提高(P<0.05);(2)各组大鼠骨组织均有TRPV6、TRPV5表达,其中山茱萸总甙干预各组TRPV6、TRPV5表达均较正常对照组及模型组增加,以中剂量组表达升高最明显,但高剂量组TRPV6/TRPV5倍比关系为最高,成骨效能最好;(3)结果说明,山茱萸总甙可调节骨质疏松大鼠骨组织TRPV6、TRPV5通道蛋白表达,改变TRPV6/TRPV5倍比关系,影响成骨细胞和破骨细胞功能,促进成骨方向的骨代谢转变及提高骨密度。BACKGROUND:Regulation of calcium channel directly affects bone metabolism,but the correlation of bone metabolism with TRPV6/TRPV5 pathways remains unclear.Cornus officinalis has been shown to treat and prevent osteoporosis.OBJECTIVE:To explore the mechanism of Cornus officinalis glycosides underlying intervening the expression of TRPV6 and TRPV5 for preventing and treating osteoporosis.METHODS:Fifty healthy 6-month-old male Sprague-Dawley rats(provide by Lanoratory Animal Center of Guangzhou University of Chiense Medicine)were selected,weighting(350±15)g.The rats were randomly divided into control(normal saline treatment),model(normal saline treatment),low-,medium,and high-dose Cornus officinalis glycosides groups.The model of osteoporosis was established by bilateral ovariectomy in the latter four groups.The administration was given gastricly,and for 12 weeks.At the end of the experiment,the bone mineral density was detected by dual energy X-ray absorptiometry.The expression levels of TRPV6 and TRPV5 in the right femur were detected by RT-PCR.RESULTS AND CONCLUSION:The bone mineral density in the Cornus officinalis glycosides groups was significantly higher than that in the model group(P<0.05).TRPV6 and TRPV5 were expressed in bone tissues of rats in each group.The expression levels of TRPV6 and TRPV5 in all the Cornus officinalis glycosides groups were higher than those in the control and model groups.The medium dose group had the highest expression levels of TRPV6 and TRPV5.The ratio of TRPV6 to TRPV5 and osteogenesis performance in the high-dose group was highest.These results imply that Cornus officinalis glycosides can promote the expression of TRPV6 and TRPV5 in bone tissue of osteoporotic rats,change the ratio of TRPV6 to TRPV5,affect the function of osteoblasts and osteoclasts,promote osteogenesis and improve bone mineral density.

关 键 词：骨质疏松 山茱萸总甙 TRPV6 TRPV5 钙离子通道 骨代谢 成骨转化 成骨功能 骨密度 山茱萸属 钙通道:组织工程 

分 类 号：R446[医药卫生—诊断学]