HPLC-DAD法同时测定蒙药森登-4汤散中7种抗风湿活性成分的含量  

Simultaneous Determination of 7 Anti-rheumatic Active Ingredients in Mongolian Medicine Sendeng-4 Decoction Powder by HPLC-DAD

作　　者：苏日塔拉图[1] 于佳琦[2] 王曦烨[2] 刘景林[2] 徐宁[2] 许良[2] Suritalatu;YU Jiaqi;WANG Xiye;LIU Jinglin;XU Ning;XU Liang(Dept. of Mongolian Medicine Preparation,the Affiliated Hospital of Inner Mongolia University for the Nationalities,Inner Mongolia Tongliao 028042,China;College of Chemistry and Chemical Engineering/Inner Mongolia Key Laboratory of Natural Products Chemistry and Functional Molecules Synthesis,Inner Mongolia University for Nationalities,Inner Mongolia Tongliao 028042,China)

机构地区：[1]内蒙古民族大学附属医院蒙药制剂室,内蒙古通辽028042 [2]内蒙古民族大学化学化工学院/天然产物化学及功能分子合成自治区重点实验室,内蒙古通辽028042

出　　处：《中国药房》2019年第2期221-225,共5页China Pharmacy

基　　金：国家自然科学基金资助项目(No.30760299;81260682;81560702)

摘　　要：目的:建立同时测定蒙药森登-4汤散中7种抗风湿活性成分儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素含量的方法。方法:采用高效液相色谱-二极管阵列检测法(HPLC-DAD)。色谱柱为Sil Green C_(18),流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1 mL/min,检测波长为238 nm,柱温为30℃,进样量为10μL。结果:儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素检测质量浓度的线性范围分别为8.590～2 290、10.56～3 901、13.00～3 958、8.815～564.2、4.030～257.8、8.130～750.5、7.075～454.2μg/mL(r≥0.999 1);检测限分别为0.429 5、0.264 0、0.325 0、0.220 4、0.201 5、0.203 2、0.176 9μg/mL,定量限分别为1.030、1.321、1.302、1.397、1.637、0.813 0、0.707 5μg/mL;精密度、稳定性(48 h)、重复性试验的RSD均小于2.0%(n=6);平均加样回收率分别为96.24%～99.28%,RSD分别为1.03%～1.63%(n=6)。结论:该方法简便、准确、重复性良好,可用于同时测定蒙药森登-4汤散中儿茶素、栀子苷、二氢杨梅素、花旗松素、芦丁、杨梅素和槲皮素的含量。OBJECTIVE:To establish a method for simultaneous determination of 7 kinds of anti-rheumatic active ingredients in Mongolian medicine Sendeng-4 decoction powder,such as catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin.METHODS:HPLC-DAD method was adopted.The determination was performed on Sil Green C18 column with mobile phase consisted of acetonitrile-0.1%phosphoric acid solution(gradient elution)at the flow rate of 1 mL/min.The detection wavelength was set at 238 nm,and column temperature was 30℃.The sample size was 10μL.RESULTS:The linear range of catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin were 8.590-2 290,10.56-3 901,13.00-3 958,8.815-564.2,4.030-257.8,8.130-750.5,7.075-454.2μg/mL(r≥0.999 1),respectively;the limits of detection were 0.429 5,0.264 0,0.325 0,0.220 4,0.201 5,0.203 2,0.176 9μg/mL,respectively;the limits of quantification were 1.030,1.321,1.302,1.397,1.637,0.813 0,0.707 5μg/mL,respectively.RSDs of precision,stability(48 h)and repetition tests were all lower than 2.0%(n=6).The average recoveries were 96.24%-99.28%(RSD=1.03%-1.63%,n=6).CONCLUSIONS:The established method is simple,accurate and reproducible,and can be used for simultaneous determination of catechin,jasminoidin,dihydromyricetin,texifolin,rutin,myricetin and quercetin in Mongolian medicine Sendeng-4 decoction powder.

关 键 词：森登-4 汤散 高效液相色谱-二极管阵列检测法 儿茶素 二氢杨梅素 花旗松素 栀子苷 芦丁 杨梅素 槲皮素 含量 

分 类 号：R917[医药卫生—药物分析学]