原子力显微镜在测定颗粒与细胞相互作用中的应用  

Application of atomic force microscopy in measuring particle-cell interactions

作　　者：宋翠[1,2] 张潇 魏炜 马光辉[1,2] SONG Cui;ZHANG Xiao;WEI Wei;MA Guanghui(State Key Laboratory Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]中国科学院大学,北京100049

出　　处：《生物加工过程》2019年第1期53-59,共7页Chinese Journal of Bioprocess Engineering

基　　金：中国科学院仪器功能开发技术创新项目(2015ga03)

摘　　要：凭借独特的尺寸效应和理化性质,纳微颗粒在生物医药领域的应用日益广泛,其与细胞的相互作用也备受关注,对其进行定量测量和机制研究愈发重要。目前,原子力显微镜(atomic force microscopy,AFM)由于具有高灵敏度(皮牛级)、高分辨率(纳米级)以及在生理环境中可进行实时检测等优势成为检测颗粒与细胞相互作用的重要工具。利用AFM检测颗粒与细胞相互作用,有助于确定作用过程中的重要参数,解释颗粒在药物递送、免疫响应和细胞力学等应用方面深层次的机制。本文中,笔者就原子力显微镜检测颗粒与细胞相互作用及其应用进行系统的综述,并对其未来的发展方向进行展望。With specific scale effects and biophysical properties,micro/nanoscale particles have been widely used in biomedical field.The interactions between particle and cell attract more and more attentions,so it is important to make quantitative measurement and mechanism research on these interactions.Nowadays,atomic force microscopy(AFM)has become an important tool for measuring the particle-cell interactions,because AFM has high force sensitivity(pN),high image resolution(nm)and can perform real time determination in physiological environment.Through AFM,it is helpful to determine the vital parameters and explain the underlying mechanism in particle-cell interaction processes,such as drug delivery,immune response and cell mechanics.This article systematically summarized the works of studying particle-cell interactions using AFM,and their subsequent applications regarding these interactions,the future direction also were discussed.

关 键 词：原子力显微镜 纳微颗粒 颗粒细胞相互作用 生物医药 

分 类 号：Q66[生物学—生物物理学]