中华蟾蜍足垫部位对植物血凝素的反应及其组织学和血液学变化  

Responsiveness to Phytohemagglutinin-P of Footpad and Changes in Histology and Hematology in Asiatic Toads(Bufo gargarizans)

作　　者：彭兵龙 张昊滨 陈可为 杨会会 连丽燕[1] 汪寅 张志强[1] PENG Bing-Long;ZHANG Hao-Bin;CHEN Ke-Wei;YANG Hui-Hui;LIAN Li-Yan;WANG Yin;ZHANG Zhi-Qiang(College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036;School of Resources and Environment,AnhuiAgricultural University,Hefei 230036,China)

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽农业大学资源与环境学院,合肥230036

出　　处：《动物学杂志》2019年第1期87-98,共12页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.31170379);2017年安徽农业大学省级大学生创新创业训练计划创新训练计划项目(No.201710364023)

摘　　要：为探究中华蟾蜍(Bufogargarizans)对植物血凝素(PHA-P)反应的最适浓度及足垫部位皮肤组织学和血液学的变化,比较了在足垫部位注射1.0、2.5和5.0g/L植物血凝素条件下的PHA-P反应,以及最适注射浓度条件下PHA-P反应高峰值时足垫部位皮肤组织和循环血中各型白细胞的百分比,血液中血清杀菌能力的变化。结果表明,中华蟾蜍对PHA-P的反应无性别差异(P> 0.05),最适注射浓度为2.5 g/L,注射后12 h达反应峰值;注射PHA-P或生理盐水的左足与注射生理盐水或不注射任何物质的右足皮肤组织相比,各型白细胞的百分比均无显著差异(P>0.05),但循环血中淋巴细胞的百分比升高(P <0.05),嗜酸性粒细胞的百分比下降(P <0.05),血清杀菌能力无明显变化(P> 0.05)。2.5 g/L的注射浓度适用于中华蟾蜍,反应高峰值与足垫部位皮肤组织中各型白细胞的动态无关,但循环血中淋巴细胞被激活、嗜酸性粒细胞功能弱化,这可能是导致中华蟾蜍PHA-P反应的主要原因。To explore the optimal injection concentration and the changes of footpad skin histology and hematology of Asiatic toads(Bufo gargarizans)after injection of phytohemagglutinin-P(PHA-P),individuals were injected at footpad with PHA-P at three concentrations(1.0 g/L,2.5 g/L and 5.0 g/L).The responsiveness to PHA-P in Asiatic toads exposed to different injection concentrations was tested and the optimal injection concentration was selected.Under the condition of the optimal PHA-P injection concentration,variations of different subtypes of leukocytes in the skin tissues of footpad position and circulation blood,as well as serum bactericidal capacity were measured when the PHA-P response reached the peak.All statistical analyses were performed using SPSS software packages(SPSS 17.0 for windows).One or two way ANOVA,or a repeated measurement one way ANOVA,or independent sample t test was used to compare the differences of body mass or percentage of different subtypes of leukocytes from skin tissues and circulation blood,or serum bactericidal capacity,or the responsiveness to PHA-P solutions among six time points.Results were presented as means±standard error,and P<0.05 was considered to be statistically significant.The results were as follows:PHA-P response did not display significant sex difference(P>0.05,Fig.2);The optimal PHA-P injection concentration was 2.5 g/L with the peak at 12 h after injection(Fig.2).No significant group differences were observed for different subtypes of leukocytes from skin tissue with injected PHA-P or saline(or without any solution)at footpad positions(P>0.05,Fig.5);At peak time,percentage of lymphocytes was increased(P<0.05,Fig.7),but percentage of eosinophils was reduced(P<0.05,Fig.7)in circulation blood,and no significant difference was found for serum bactericidal capacity(Fig.8).The optimal 2.5 g/L PHA-P concentration was appropriate for Asiatic toads.The peak of PHA-P response was not correlated with the dynamic changes of any subtype of leukocyte in the footpad skin tissues,but ab

关 键 词：中华蟾蜍 足垫 血清杀菌能力 淋巴细胞 嗜酸性粒细胞 

分 类 号：Q955[生物学—动物学]