lncRNA在冠心病氯吡格雷抵抗患者中的差异性表达  

Differential expression of lncRNA in patients with coronary artery disease plus clopidogrel resistance

作　　者：谢文剑[1] 黄贝贝 殷蒨 陈绍良[1] XIE Wenjian;HUANG Beibei;YIN Qian;CHEN Shaoliang(Department of Cardiology,Nanjing Hospital Affi liated to Nanjing Medical University,Nanjing 210006,China;General Clinical Research Center,Nanjing Hospital Affi liated to Nanjing Medical University,Nanjing 210006,China)

机构地区：[1]南京医科大学附属南京医院心内科,南京210006 [2]南京医科大学附属南京医院临床研究中心,南京210006

出　　处：《中南大学学报（医学版）》2019年第1期9-13,共5页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81473286);江苏省科技发展计划(BL2013001)~~

摘　　要：目的:探讨长链非编码RNA(long non-coding RNA,lncRNA)在冠状动脉粥样硬化性心脏病(以下简称"冠心病")氯吡格雷抵抗患者中的差异性表达。方法:收集经皮冠状动脉介入治疗(percutaneous coronary intervention,PCI)术后口服氯吡格雷的冠心病患者的临床资料和血液标本,根据血小板聚集率将患者分为氯吡格雷敏感组和抵抗组。两组患者各取5例,采用lncRNA芯片筛选;并另外分别取34例进行real-time RT-PCR验证。结果:lncRNA芯片筛选显示:氯吡格雷抵抗组白细胞中有11个lncRNA上调,8个lncRNA下调。Real-time RT-PCR验证显示:与氯吡格雷敏感组相比,氯吡格雷抵抗组的外周血白细胞中有2个lncRNA(NONHSAT083775.2和NONHSAT107804.2)上调,1个lncRNA(NONHSAT133455.2)下调,与芯片分析结果一致。结论:NONHSAT083775.2和NONHSAT107804.2的上调以及NONHSAT133455.2的下调与氯吡格雷抵抗有关。Objective:To explore diff erential expression of long non-coding RNA(lncRNA)in patients with coronary artery disease plus clopidogrel resistance.Methods:Patients underwent percutaneous coronary intervention(PCI)and treated with clopidogrel were recruited,and their clinical data and blood samples were collected.Patients were divided into a clopidogrel sensitive group and a clopidogrel resistance group according to platelet aggregation rate.lncRNA microarray and real-time RT-PCR were performed in 5 and 34 patients in each group,respectively.Results:lncRNA microarray showed that 11 lncRNAs in peripheral leukocytes were up-regulated and 8 lncRNAs were down-regulated in clopidogrel resistant group.Real-time PCR indicated that two lncRNAs(NONHSAT083775.2 and NONHSAT107804.2)in leukocytes were up-regulated and one lncRNA(NONHSAT133455.2)was down-regulated in the clopidogrel resistant group compared with the clopidogrel sensitive group,consistent with the results of lncRNA microarray.Conclusion:Clopidogrel resistance is associated with the up-regulation of lncRNA NONHSAT083775.2 and NONHSAT107804.2 and the down-regulation of NONHSAT133455.2.

关 键 词：冠状动脉粥样硬化性心脏病 氯吡格雷抵抗 长链非编码RNA 

分 类 号：R541.4[医药卫生—心血管疾病]