球孢白僵菌发酵液抗人体致病细菌活性研究  

Antimicrobial Studies on Beauveria bassiana against Human Pathogenic Bacteria

作　　者：李娟[1,2] 虞泓 王毅[2] LI Juan;YU Hong;WANG Yi(Yunnan University,Kunming Yunnan 650091,P.R.China;The Key Laboratory of Forest Plants Cultivation and Utilization,The Key Laboratory of Rare and Endangered Forest Plants of State Forestry Administration,Yunnan Academy of Forestry,Kunming Yunnan 650201,P.R.China)

机构地区：[1]云南大学,云南昆明650091 [2]云南省林业科学院云南省森林植物培育与开发利用重点实验室国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南昆明650201

出　　处：《西部林业科学》2019年第1期18-22,28,共6页Journal of West China Forestry Science

基　　金：国家自然科学基金项目(31860177);国家自然科学基金地区项目(31760011);云南省面上基金(2016FB055);云南省林业科学院创新基金项目(QN2018-01);中央引导地方科技发展专项资金项目(KC1610530)

摘　　要：为了探究球孢白僵菌YFCCFQ001发酵液的抗菌活性以及不同培养基对其抗菌效果的影响,用乙酸乙酯萃取球孢白僵菌YFCCFQ001发酵液,旋干后得到提取物,采用抑菌圈法测定其对6种耐药性人体致病细菌以及8种普通人体致病细菌的抗菌活性,并通过单菌多产物策略,改变培养基碳氮源,检测抗菌效果的变化。结果表明:球孢白僵菌YFCCFQ001提取物对4种耐药性致病细菌(溶血性葡萄球菌、大肠埃希菌、粪肠球菌、金黄色葡萄球菌)和8种普通致病细菌均有抗菌活性(其中关于球孢白僵菌对副溶血性弧菌、短小芽孢杆菌、无乳链球菌、福氏志贺氏菌、乙型副伤寒沙门氏菌的抗菌活性未见文献报道)。不同氮源对其抗菌活性影响显著,其中胰蛋白胨抗菌效果最好,对3种耐药性致病细菌和6种普通致病细菌均有活性。研究发现了球孢白僵菌对耐药性致病细菌的抗菌活性,以及不同培养基对球孢白僵菌发酵液抗菌活性的影响,为真菌中新型抗菌天然产物的寻找和利用提供新的思路。In order to explore the antibacterial activity of extracts of Beauveria bassiana(YFCCFQ001)and effect of different medium on antibacterial activity,inoculating inhibition zone was used to evaluate the antimicrobial activity of ethyl acetate extracts of B.bassiana against 6 resistant pathogenic bacteria and 8 common human pathogenic bacteria,and one strain-many compounds(OSMAC)strategy was adopted to evaluate the effect of different carbon sources,nitrogen sources and inoculation on antibacterial activity.The results showed that the extracts of B.bassiana had significant antibacterial activity against 4 resistant pathogenic bacteria(Straphylococcus haemolyticus,Staphylococcus aureus,Enterococcus faecalis,Escherichia coli)and 8 common pathogenic bacteria(Beauveria bassiana against Vibrio parahaemolyticus,Bacillus pumilus,Streptcococcus agalactiae,Shigella flexneri,Salmonella paratyphi were the firstly reported).Different nitrogen sources had significant effects on antibacterial activity,and the extracts from tryptone had the best antibacterial effect that against 3 resistant pathogenic bacteria and 6 common pathogenic bacteria.The antibacterial activity of B.bassiana against resistant pathogenic bacteria and the effect of different medium on antibacterial activity was found,and it is beneficial to the development of novel antimicrobial compounds against human pathogenic bacteria.

关 键 词：球孢白僵菌 OSMAC策略 抗菌活性 耐药性致病细菌 人体致病细菌 

分 类 号：Q939.9[生物学—微生物学]