亚硒酸钠对小鼠脾脏淋巴细胞体外增殖及细胞因子分泌的影响  

Effects of Sodium Selenite on the Proliferation of Splenic Lymphocytes and Cytokine Secretion in vitro of Mice

作　　者：李冰心 李婉雁 田允波 曹楠 许丹宁 LI Bing-xin;LI Wan-yan;TIAN Yun-bo(Guangdong Province Key Laboratory of Waterfowl Healthy Breeding,Guangzhou,Guangdong 510225;College of Animal Science & Technology,Zhongkai University of Agriculture and Engineering,Guangzhou,Guangdong 510225)

机构地区：[1]广东省水禽健康养殖重点实验室,广东广州510225 [2]仲恺农业工程学院动物科技学院,广东广州510225

出　　处：《安徽农业科学》2019年第2期80-82,共3页Journal of Anhui Agricultural Sciences

基　　金：广东省"创新强校工程"基础研究重大项目(2017KZDXM046);广东省科技计划项目(2017B020202010)

摘　　要：[目的]研究亚硒酸钠对小鼠脾脏淋巴细胞体外增殖及脾脏淋巴细胞中细胞因子分泌的影响,进而探讨亚硒酸钠调节机体免疫可能的作用途径。[方法]从BALB/c小鼠脾脏中分离淋巴细胞,采用MTS法检测不同浓度(0、10^(-8)、10^(-7)、10^(-6)和10^(-5) mol/L)的亚硒酸钠培养基培养48 h后的淋巴细胞增殖率并筛选出最适浓度的亚硒酸钠进行脾脏淋巴细胞培养,最后采用ELISA法检测培养48 h后培养液中IFN-γ、IL-1β、IL-4和IL-6的分泌量。[结果]与对照组相比,10^(-7) mol/L亚硒酸钠处理使淋巴细胞增殖率显著提高,且培养液中IFN-γ、IL-1β、IL-4和IL-6的分泌量分别为对照组的10.34、2.15、1.06和6.68倍(P<0.05)。[结论]亚硒酸钠可能通过调节脾脏淋巴细胞增殖和细胞因子的分泌来调节机体免疫功能。[Objective]To study the effects of sodium selenite on the spleen lymphocyte’s proliferation and the cytokines secretion of mice in vitro,and explore the possible immune regulation ways of sodium selenite.[Method]The lymphocyte was isolated from the spleen of BALB/c mice.MTS method was used to detect the proliferation rate of lymphocytes cultured in sodium selenite medium with different concentrations (0,10^-8,10^-7,10^-6 and 10^-5 mol/L) after 48 h and screen out the optimum concentration of sodium selenite for spleen lymphocyte culture.The secretion quantity of IFN-γ,IL-1β,IL-4 and IL-6 in culture supernatant after culturing 48 h were measured by ELISA.[Result]The proliferation rate of lymphocytes treated by 10^-7 mol/mL sodium selenite was significantly increased compared with that in control group,and the secretion quantity of IFN-γ,IL-1β,IL-4 and IL-6 in culture supernatant increased by 10.34,2.15,1.06,and 6.68 times,respectively.[Conclusion]Sodium selenite may regulate the body immunity by regulating spleen lymphocyte’s proliferation and secretion of cytokines.

关 键 词：亚硒酸钠 小鼠 淋巴细胞 增殖率 细胞因子 

分 类 号：S852.4[农业科学—基础兽医学]