芦丁对梗阻性肾病大鼠肾脏氧化应激损伤及Nrf2/HO-1通路的影响  

Effects of rutin on kidney oxidative stress and Nrf2/HO-1 signaling pathway in rats with obstructive nephropathy

作　　者：王斌[1] 赵明[2] 陈志勇[1] 赖永通[1] 郭雪坤[1] 黄先恩[1] 赵国志[1] 蔡瑞明[1] 林民专[1] WANG Bin;ZHAO Ming;CHEN Zhiyong;LAI Yongtong;GUO Xuekun;HUANG Xian′en;ZHAO Guozhi;CAI Ruiming;LIN Minzhuan(Department of Organ Transplantation,the Third Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510150,China;Department of Organ Transplantation,Zhujiang Hospital of Southern Medical University,Guangzhou,Guangdong 510282,China)

机构地区：[1]广州医科大学附属第三医院器官移植科,广州510150 [2]南方医科大学珠江医院器官移植科,广州510282

出　　处：《重庆医学》2019年第2期212-216,共5页Chongqing medicine

基　　金：广东省科学技术厅科技计划项目(2016A020220014)

摘　　要：目的探讨芦丁对梗阻性肾病大鼠肾脏氧化应激损伤及Nrf2/HO-1通路的影响。方法雄性Wistar大鼠分为4组:假手术组、芦丁治疗假手术组、模型组、芦丁治疗模型组。术后24h,芦丁治疗假手术组和芦丁治疗模型组给予芦丁[100mg·kg-1·d-1溶于1%羧甲基纤维素钠(CMC-Na)溶液]灌胃,其他两组给予等体积的1%CMC-Na溶液灌胃。术后2周处死全部大鼠,取梗阻侧肾组织测定脂质过氧化产物丙二醛(MDA)水平、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)的活性,用苏木素-伊红(HE)染色观察各组大鼠肾脏病理改变,免疫组织化学方法检测肾组织内血红素加氧酶-1(HO-1)蛋白的表达水平。Western blot方法检测肾组织中核因子E2相关因子2(Nrf2)和HO-1蛋白表达水平。结果与假手术组比较,模型组肾脏病理损害明显加重,肾组织内MDA水平明显增加(P<0.05),SOD及GSH-Px活性明显降低(P<0.05),Nrf2与HO-1蛋白表达水平明显升高(P<0.05)。与模型组比较,芦丁治疗模型组肾脏病理损害明显减轻,肾组织内MDA水平明显减少(P<0.05),SOD及GSH-Px活性明显升高(P<0.05),Nrf2与HO-1蛋白表达水平进一步升高(P<0.05)。结论芦丁可减轻梗阻性肾病大鼠肾脏氧化应激损伤,此效应可能是通过介导Nrf2/HO-1信号通路实现。Objective To explore the effects and mechanisms of rutin on kidney oxidative stress injury and Nrf2/HO-1 signaling in rats with obstructive nephropathy.Methods Male Wistar rats were divided into 4 groups:the sham group,the rutin+sham group and the operated group,the rutin+operated group.Rats in the rutin+sham group and the rutin+operated group were intragastrically administered with rutin[100 mg·kg^-1·d^-1 dissolved in 1%carboxymethylcellulose-Na(CMC-Na)buffer].The other two groups were given an equal volume of 1%CMC-Na solution by gavage.2 weeks after surgery,all rats were sacrificed and the obstructed kidneys were harvested.Level of malondialdehyde(MDA),activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in the obstructed kidneys were measured.Hematoxylin and eosin(HE)staining was performed to observe the pathological changes of the obstructed kidney.Expression of heme oxygenase-1(HO-1)in renal tissues were analyzed by immunohistochemistry.Western blot was used to detect the expression of nuclear factor-E2-related factor 2(Nrf2)and HO-1 protein.Results Compared with the sham group,rats in the operated group showed significant kidney pathological injury,the level of MDA increased(P<0.05).The expression of Nrf2 and HO-1 increased,and the activity of SOD and GSH-Px decreased significantly(P<0.05).Comparing with the operated group,the rutin+operated group showed that rutin ameliorated histological injury obviously,decreased the level of MDA,increased the activities of SOD and GSH-Px,and enhanced the expression of Nrf2 and HO-1 in rats.Conclusion Rutin could ameliorate oxidative stress in rats with obstructive nephropathy via Nrf2/HO-1 signaling pathway possibly.

关 键 词：芦丁 梗阻性肾病 氧化性应激 血红素加氧酶-1 核因子E2相关因子2 

分 类 号：R692.2[医药卫生—泌尿科学]