细鳞斜颌鲴5种组织2种同工酶的电泳分析  

Electrophoretic analysis for two kinds of isozymes expressed in 5 tissues of Xenocypris microlepis

作　　者：张涛[1] 甘金华[1] 周剑光[1] 张林[1] 陈建武[1] 何力[1] ZHANG Tao;GAN Jinhua;ZHOU Jianguang;ZHANG Lin;CHEN Jianwu;HE Li(Laboratory of Quality & Safety Risk Assessment for Aquatic Products(Wuhan), Ministry of Agriculture; Freshwater Fish Germplasm Quality Supervision and Testing Center, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223,China)

机构地区：[1]农业部水产品质量安全风险评估实验室(武汉)农业部淡水鱼类种质监督检验测试中心中国水产科学研究院长江水产研究所,武汉430223

出　　处：《中国渔业质量与标准》2019年第1期10-17,共8页Chinese Fishery Quality and Standards

基　　金：国家农产品质量安全风险评估重大专项(GJFP201700902)

摘　　要：为了解细鳞斜颌鲴(Xenocypris microlepis)的生化遗传特征,并以此为基础为其种质资源鉴定提供理论依据,本研究采用不连续聚丙烯酰胺凝胶垂直板电泳对细鳞斜颌鲴5种组织(心脏、晶状体、肌肉、肾脏和肝脏)的乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)进行分析。结果表明:所测样本鱼的心脏、晶状体、肌肉、肾脏和肝脏中的LDH酶带数分别为:6～8、5、2～4、5和4～6条; MDH酶带数分别为:7～8、5～6、7～8、3～4和4条;细鳞斜颌鲴的LDH同工酶和MDH同工酶具有组织特异性,其心脏、肾脏和肝脏MDH分为上清液型(s-MDH)和线粒体型(m-MDH),肌肉和晶状体组织中未见上清液型MDH(s-MDH)。研究认为晶状体组织LDH可作为鉴定细鳞斜颌鯝种质特征的生化遗传参数。本研究可为鉴定细鳞斜颌鲴种质、制定其种质标准提供参考依据。This paper was to study the morphological and biochemical genetics characteristics of Xenocypris microlepis so as to provide theoretical basis for their germplasm resources identification. By using the vertical polyacrylamide gel electrophoresis, lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isozymes from five tissues (heart, eye, muscle, kidney and liver) were analyzed . The results showed that: the band numbers of LDH isozymes expressed in heart, eye, muscle, kidney and liver were 6-8, 5, 2-4, 5 and 4-6, respectively, and that of MDH were 7-8, 5-6, 7-8, 3-4 and 4, respectively. These two kinds of isozymes from Xenocypris microlepis presented tissue specificity. Briefly, MDH isozymes expressed in heart, kidney and liver of Xenocypris microlepis were divided into supernatant type (s-MDH) and mitochondria type (m-MDH). The supernatant type of malate dehydrogenase (MDH) isozymes could not be detected in muscle and eyes. And lactate dehydrogenase (LDH) isozymes expressed in eyes can be used as the biochemical genetic marker of Xenocypris microlepis. This research provided a reference basis for the establishment of the germplasm standards and the germplasm identification of Xenocypris microlepis.[Chinese Fishery Quality and Standards,2019,9(1):10-17].

关 键 词：细鳞斜颌鲴 乳酸脱氢酶 苹果酸脱氢酶 同工酶电泳 特征生化遗传参数 组织特异性 种质资源鉴定 

分 类 号：S917.4[农业科学—水产科学]