驱风止痛散对酵母联合腺嘌呤致痛风性肾病的作用及机制  

Effect and mechanism of QuFengZhiTongSan on gouty nephropathy induced by yeast combined with adenine

作　　者：郭思彤 许立拔[1] 梁小玲 黄菊 刘丽敏[1] GUO Sitong;XU Liba;LIANG Xiaoling;HUANG Ju;LIU Limin(Pharmaceutical College of Guangxi Medical University,Nanning,Guangxi 530021,China)

机构地区：[1]广西医科大学药学院,广西南宁530021

出　　处：《现代医药卫生》2019年第3期342-344,349,共4页Journal of Modern Medicine & Health

基　　金：广西壮族自治区教育厅高校科学技术研究项目(KY2015YB059)

摘　　要：目的研究驱风止痛散对酵母联合腺嘌呤致痛风性肾病模型大鼠肾功能的保护作用及机制。方法将60只大鼠随机分为6组:空白组、模型组、别嘌醇组(0.01g/kg),驱风止痛散低、中、高剂量组(1.60、3.20、6.40g/kg),每组10只。每天上午,空白组大鼠给予等体积纯净水,其余各组大鼠灌胃酵母粉腺嘌呤混悬液(酵母粉10g/kg、腺嘌呤0.01g/kg)造模;每天下午,空白组和模型组大鼠给予等体积纯净水,其余各组大鼠灌胃给予相应药液,连续造模兼给药28d。检测各组大鼠血清中尿酸(SUA)、肌酐(SCr)、尿素氮(BUN)、转化生长因子-β1(TGF-β1)、白细胞介素-1β(IL-1β)的含量。观察各组大鼠肾脏组织切片中病理变化。结果与空白组比较,其他各组大鼠SUA、SCr水平均明显升高,差异均有统计学意义(P<0.01或0.05);模型组大鼠血清BUN水平明显升高,差异均有统计学意义(P<0.01)。与模型组比较,别嘌醇组及驱风止痛散高、中、低剂量组大鼠SUA、SCr、BUN水平均明显降低,差异均有统计学意义(P<0.01或0.05),且呈一定的剂量依赖性。与空白组比较,其他各组大鼠血清TGF-β1、IL-1β水平明显升高,差异均有统计学意义(P<0.01)。与模型组比较,别嘌醇组大鼠血清TGF-β1、IL-1β水平及驱风止痛散高、中、低剂量组大鼠血清TGF-β1水平均降低,差异均有统计学意义(P<0.01)。驱风止痛散高、中、低剂量组大鼠血清TGF-β1水平高于别嘌醇组,中、低剂量组大鼠血清IL-1β水平显著高于别嘌醇组,差异均有统计学意义(P<0.01)。病理分析提示,别嘌醇组和驱风止痛散各剂量组肾小管扩张和间质纤维化有所改善,炎症细胞浸润有所减少。结论驱风止痛散对酵母联合腺嘌呤诱导的痛风性肾病大鼠的肾功能有保护作用。Objective To study on the protective effect and mechanism of QuFengZhiTongSanon(QFZTS)renal function in rats model of gouty nephropathy induced by yeast and adenine.Methods Sixty SD rats were randomly divided into six groups with ten rats per group:blank group,model group,allopurinol group(0.01 g/kg),QFZTS low dose group(1.60 g/kg),QFZTS middle dose group(3.20 g/kg)and QFZTS high dose group(6.40 g/kg).Every morning,the rats of the blank group were given pure water of equal volume,the rats of the other group were given the suspension of yeast with adenine(yeast 10 g/kg,adenine 100 mg/kg)by intragastric perfusion;Every afternoon,the rats of the blank group and model group were given pure water of equal volume,the rats of the other group were given the corresponding solution by intragastric perfusion,modeling and administration were lasted 28 days.Serum uric acid(SUA),serum creatinine(SCr),blood urea nitrogen(BUN),transforming growth factor-β1(TGF-β1),interleukin-1β(IL-1β)of rats in each group were examined.The pathological changes in renal tissue sections of rats in each group were observed.Results Compared with blank group,the SUA and SCr levels were significantly increased in all the other groups,with statistically significant differences(P<0.01 or 0.05);the BUN level was significantly increased in the model group,and the difference was statistically significant(P<0.01).Compared with the model group,the levels of SUA,SCr and BUN were significantly reduced in the allopurinol group and the QFZTS groups with high,medium and low doses,and the differences were statistically significant(P<0.01 or 0.05),with a certain dose dependence.Compared with the blank group,the TGF-β1 and IL-1βlevels were significantly increased in other groups,with statistically significant differences(P<0.01).Compared with the model group,the TGF-β1 and IL-1βlevels in the allopurinol group and the TGF-β1 level in the QFZTS group with high,medium and low doses were all decreased,and the differences were statistically significant(P<0.01).

关 键 词：痛风 肾疾病 尿酸/血液 肌酸酐/血液 肾/病理学 驱风止痛散 痛风性肾病 炎症因子 

分 类 号：R285.5[医药卫生—中药学]