甜瓜果长基因fl与性别表达基因a的遗传分析及定位  

Genetic Analysis and Primary Localization of Fruit Length Gene fl and Sex Expression gene a in Melon (Cucumis melo L.)

作　　者：范文林[1] 王贤磊[1] 李群[1] 俞志杰 李冠[1] FAN Wen-lin;WANG Xian-lei;LI Qun;YU Zhi-jie;LI Guan(The College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《新疆农业科学》2018年第10期1765-1774,共10页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区自然科学基金项目(2016D01C066)~~

摘　　要：【目的】研究甜瓜2号连锁群中果长基因fl与性别表达基因a的连锁关系。分析果实长度和性别表达类型(雄花两性花同株、雌雄异花同株)的遗传规律,定位两性状基因。【方法】以圆形甜瓜、雄花两性花同株品种西州蜜为父本,长形甜瓜、雌雄异花同株品种蛇瓜为母本杂交产生的160个BC_1(Back Cross回交群体)单株为作图群体,研究BC_1群体中果实长度和性别类型的分布,对二者进行遗传分析。利用集团分离分析法(Bulked Segregant Analysis, BSA),用甜瓜2号连锁群上的48个SSR分子标记,对甜瓜果实长度和花性型性状进行多态性标记的筛选,对两性状基因定位。【结果】甜瓜果实长度符合数量性状的遗传特点;雄花两性花同株与雌雄异花同株可能受双基因遗传控制。通过连锁分析,将果长基因fl定位于2号连锁群的标记SSR247159和标记SSR252089之间,遗传距离为3 cM;将性别表达基因a定位于标记SSR227156和标记CMGA36/SSR235092之间,遗传距离为3.59 cM;两区间之间的遗传距离为0。【结论】在甜瓜西州蜜和蛇瓜的BC_1群体中,将果实长度基因fl和性别表达基因a的初步定位于不同标记区间内,证明二者不是同一基因。【Objective】To identify the linkage relationship between fruit length gene fland sex expression gene a on LG2(Linkage Group 2)in melon,160 BC 1 plants derived from the cross between the andromonoecious melon line'Xizhoumi'with round fruit and the monoecious melon line'snake melon'with oblong fruit were used to investigate the genetic mechanism and perform the primary gene localization for fruit length and sex expression type(andromonecious,monecious).【Method】Basing on the phenotypic frequency distribution of the above two traits,the genetic mechanism was analyzed.By BSA(Bulked Segregant Analysis),48 SSR markers on LG2 were screened to obtain the polymorphism SSR markers for the two traits.And the hermaphroditic genes were primarily localized.【Result】The result of genetic analysis showed that the fruit length conformed to the QTL genetic mechanism,and sex expression type(andromonecious,monecious)was possibly controlled by the two genes.The result of linkage analysis showed that fruit length gene fl was localized between marker SSR247159 and marker SSR252089 on LG2,the genetic distance was 3 cm;sex expression gene a was localized between marker SSR227156 and marker CMGA36/SSR235092 on LG2,the genetic distance was 3.59 cm;the genetic distance between the two intervals was 0.6 cm.【Conclusion】In the BC 1 populations derived from the cross between'Xizhoumi'and'snake melon',the fruit length gene fl and the sex expression gene a were localized in different marker regions,which proved that the two genes were not the same gene.

关 键 词：甜瓜 果长基因 性别表达基因a 遗传定位 

分 类 号：S[农业科学]