广西贵港地区猪繁殖与呼吸障碍综合征调查分析  

Investigation and Analysis of Porcine Reproductive and Respiratory Syndrome in Guigang,Guangxi

作　　者：张书祥 何奇松[2] 孔子荣 郭建刚[2] 周庆安 黄胜斌[2] 韩银华[2] 熊毅[2] ZHANG Shu-xiang;HE Qi-song;KONG Zi-rong;GUO Jian-gang;ZHOU Qing-an;HUANG Sheng-bin;HAN Yin-hua;XIONG Yi(College of Animal Science and Technology,Guangxi University,Guangxi Nanning 530004,China;Guangxi Center for Animal Disease Prevention and Control,Guangxi Nanning 530001,China)

机构地区：[1]广西大学动物科学技术学院,广西南宁530004 [2]广西动物疫病预防控制中心,广西南宁530001

出　　处：《西南农业学报》2019年第1期208-213,共6页Southwest China Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2018YED0500800)

摘　　要：【目的】了解广西贵港地区猪繁殖与呼吸障碍综合征的感染情况,为广西贵港地区猪繁殖与呼吸障碍综合征的监督及防控工作提供参考。【方法】通过对Nsp2基因的分析,合成新的特异性诊断引物,并利用RT-PCR的方法对广西贵港地区111个规模猪场采集的1865份扁桃体同时进行高致病性猪繁殖与呼吸障碍综合征和经典猪繁殖与呼吸障碍综合征的分子流行病学调查。【结果】25个猪场检出92份猪繁殖与呼吸障碍综合征病毒,场点总阳性率为22. 5%,样品总阳性率为4. 93%。其中9个猪场检测出24份高致病性猪繁殖与呼吸障碍综合征病毒,场点阳性率为8. 10%,样品阳性率为1. 29%; 18个猪场检测出68份经典猪繁殖与呼吸障碍综合征病毒,场点阳性率为16. 2%,样品阳性率为3. 65%; 2个猪场同时检测出高致病性猪繁殖与呼吸障碍综合征病毒和经典猪繁殖与呼吸障碍综合征病毒。【结论】广西贵港地区规模猪场感染猪繁殖与呼吸障碍综合征的现象较为严重。【Objective】To provide a reference for strengthening the supervision and prevention and control of this disease,the prevalence of porcine reproductive and respiratory syndrome in Guigang,Guangxi was studied.【Method】Through the analysis of Nsp2 gene,new specific diagnostic primers were synthesized,and the molecular epidemiology of two types of porcine reproductive and respiratory syndrome-highly pathogenic and classic,were investigated simultaneously using 1865 tonsils collected from 111 large-scale pig farms in Guigang,Guangxi.【Results】92 cases of porcine reproductive and respiratory syndrome virus were detected in 25 pig farms,with a positive rate of 22.5%in the site and 4.93%in the sample.24 samples of highly pathogenic porcine reproductive and respiratory syndrome virus infection were detected in 9 pig farms,with a positive rate of 8.10%in the site and a positive rate of 1.29%in the samples.68 samples of classic reproductive and respiratory syndrome virus infection were detected in 18 pig farms,with a positive rate of 16.2%in the site and a positive rate of 3.65%in the samples.Highly pathogenic and classic porcine reproductive and respiratory syndrome virus infection samples were simultaneously detected in 2 pig farms.【Conclusion】The infection of porcine reproductive and respiratory syndrome in large-scale pig farms in Guigang,Guangxi is serious.

关 键 词：猪繁殖与呼吸障碍综合征 扁桃体 NSP2 感染 调查 

分 类 号：S828[农业科学—畜牧学]