福建省2015年本地登革热病例的病原学特征  

Etiological characterization of indigenous dengue cases in Fujian province,2015

作　　者：张拥军[1,2] 吴生根 王金章[1,2] 游丽斌 阚乃鹏[1,2] 翁育伟 ZHANG Yong-jun;WU Sheng-gen;WANG Jin-zhang;YOU Li-bin;KAN Nai-peng;WENG Yu-wei(Fujian Center for Disease Control and Prevention,Fuzhou 350001,China;Fujian Provincial Key Laboratory of Zoonosis Disease Research,Fuzhou 350001,China)

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建省人兽共患病重点实验室,福州350001

出　　处：《中国人兽共患病学报》2019年第1期28-33,共6页Chinese Journal of Zoonoses

基　　金：福建省科技厅引导性项目(No.2016Y0011);福建省卫计委青年科研课题(No.2018-1-22);福建省医学创新课题(No.2015-CXB-13)联合资助;Fujian Provincial Health and Family Planning Commission~~

摘　　要：目的分析2015年福建省本地登革热病例部分登革病毒流行株的基因组序列,调查相关病毒株之间的遗传联系,为福建省登革热防控提供病原学证据。方法采集急性期患者血清,实时荧光RT-PCR检测登革病毒RNA,阳性者分离病毒,扩增病毒基因组并测序,以病毒全长编码区序列进行种系发生分析。结果 2015年福建省先后出现登革1型(DENV1)和登革2型病毒(DENV2)引起的本地暴发疫情,共发生4起聚集性病例,报告41例。分离到DENV1毒株9株,DENV2毒株8株。种系发生分析显示,9株DENV1高度相似,同属于基因1型(G1),提示其共同的输入来源可能为斯里兰卡;而8株DENV2病毒虽然同属于大都市基因型,却分为差异较大的两簇,表明莆田与福州两地疫情相关毒株的遗传关联度较低,可能分别来自马来西亚和印度。结论 2015年共出现4起聚集性本地登革热疫情,部分患者病毒分离株的病原学特征表明,福建省2015年本地登革热暴发疫情存在多个输入来源。Introduction of dengue viruses from dengue-endemic regions may lead to indigenous outbreaks in regions with high density of mosquito vectors.During mid-September to mid-October in 2015,a total of 41 indigenous dengue cases emerged in 4 clusters were notified in Fujian province,China.In this study,the etiological characterization of clinical isolates linked to these outbreaks was investigated.It was revealed by phylogenetic analyses on the full-length coding sequences that the 9 dengue serotype 1(DENV1)isolates,belonging to genotype 1(G1),showed high similarity in nucleotide sequences,and that the 8 dengue serotype 2(DENV2)isolates showed distinct variations within the genotype of Cosmopolitan.According to the phylogenetic trees,DENV1 strains associated with clusters 2 and 4 were likely originated from Sri Lanka,while Malaysia and India were sources of the DENV2 strains associated with Cluster 1 and Cluster 3,respectively.Therefore,these results suggested that multiple sources of introduction caused the indigenous dengue outbreaks in Fujian province,2015.

关 键 词：登革热 病原学 基因组 序列分析 

分 类 号：R373.3[医药卫生—病原生物学]