不同配伍比例刺五加和树舌的镇静催眠作用  

Sedative and Hypnotic Effects of Different Compatibility Ratios of Acanthopanax and Tree Tongue

作　　者：卢铭 王凤芝[2] 李琪[2] 王丽红[1] LU Ming;WANG Feng-zhi;LI Qi(School of Pharmac,Jiamusi University,Jiamusi,Heilongjiang 154007;School of Medicine,Jiamusi University,Jiamusi,Heilongjiang 154007)

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]佳木斯大学临床医学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2019年第3期150-153,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省北药及功能食品优势学科;中-乌农林技术开发与应用国际合作联合实验室项目

摘　　要：[目的]探究不同配伍比例刺五加-树舌灵芝醇提物对小鼠镇静催眠作用的影响。[方法]不同配伍比例的刺五加和树舌灵芝用75%乙醇作为溶剂,料液比为1∶20,利用冷凝回流提取1.5 h后得各配伍比例的乙醇提取物;利用差热分析仪对得到的不同配伍比例的乙醇提取物进行分析;应用苯巴比妥钠所致小鼠睡眠的方法,研究刺五加和树舌灵芝乙醇提取物改善睡眠的量效关系。[结果]差热分析结果显示,刺五加和树舌灵芝配比为1∶5和5∶1时,2种药物配伍后的相互作用明显。镇静催眠作用结果显示,配伍比例为1∶5、3∶1、4∶1、5∶1小鼠的体重依次显著增大(P<0.05);配伍比例为1∶1、1∶2、2∶1、1∶3、1∶4、1∶5、3∶1、4∶1、5∶1的给药组抑制小鼠的活动次数(P<0.05)依次增大;阈下剂量苯巴比妥钠对小鼠镇静催眠试验结果显示,所有给药组均有镇静催眠效果;阈上剂量苯巴比妥钠对小鼠镇静催眠试验结果显示,配伍比例为1∶1、1∶2、2∶1、1∶3、1∶4、1∶5、3∶1、4∶1、5∶1的给药组镇静催眠作用(P<0.05)依次增大。[结论]刺五加-树舌灵芝配比为5∶1的醇提物对小鼠的镇静催眠作用效果最好,其作用优于单味药刺五加组和单味药树舌灵芝组。[Objective]To investigate the effects of different compatibility ratios of ELeutherococcus senticosus and Ganoderma applanatum on the sedative and hypnotic effects of mice.[Method]Different compatibility ratios of ELeutherococcus senticosus and Ganoderma applanatum were treated with 75%ethanol as solvent.The ratio of material to liquid was 1∶20.Each ethanol extract was obtained after reflux extraction for 1.5 h.Different comparable ethanol extracts were analyzed by differential thermal analyzer;the method of phenobarbital sodium-induced sleep in mice was used to study the dose-effect relationship of Acanthopanax senticosus and Ganoderma lucidum ethanol extract to improve sleep.[Result]The results of differential thermal analysis showed that the compatibility of the two drugs was obvious when the ratio of ELeutherococcus senticosus and Ganoderma applanatum was 1∶5 and 5∶1.The results of sedative and hypnotic effects showed that the body weights of the 1∶5,3∶1,4∶1 and 5∶1 mice were in turn increased significantly(P<0.05);the compatibility ratio was 1∶1,1∶2,and 2∶1,1∶3,1∶4,1∶5,3∶1,4∶1,5∶1 administration group inhibited the number of mice activity(P<0.05)in turn increased;subthreshold dose of phenobar.The results of sedative and hypnotic test of mice treated with Betulin showed sedative and hypnotic effects in all groups.The sedative and hypnotic test results of phenobarbital on suprathreshold mice showed that the proportion of phenobarbital was 1∶1 and 1∶2.The sedative and hypnotic effects of the 2∶1,1∶3,1∶4,1∶5,3∶1,4∶1 and 5∶1 administration groups increased sequentially(P<0.05).[Conclusion]The alcohol extracts of ELeutherococcus senticosus and Ganoderma applanatum with a ratio of 5∶1 have the best sedative and hypnotic effect on mice.Its effect is better than the single-flavored medicine acanthopanax group and the single-flavored medicine tongue and tongue group.

关 键 词：刺五加 树舌灵芝 翻正反射 醇提物 镇静催眠 

分 类 号：R285[医药卫生—中药学]