蜂胶黄酮作为PPV灭活疫苗佐剂活性的研究  

Adjuvant activity of propolis flavone for PPV vaccine

作　　者：翟晓虎[1] 徐传芬 张玲玲 贺卫华[1] 马霞[4] 杨俊花[5] ZHAI Xiao-hu;XU Chuan-fen;ZHANG Ling-ling;HE Wei-hua;MA Xia;YANG Jun-hua(Jiangsu Agri-Animal Husbandry Vocational College,Taizhou 225300,China;Forestry Station in Wennan Town of Xintai,Tai’an 271200,China;Zhengzhou Institute of City Landscape Architecture Science,Zhengzhou 450000,China;Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;Institute for Agri-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]江苏农牧科技职业学院,泰州225300 [2]新泰市汶南镇林业站,泰安271200 [3]郑州市城市园林科学研究所,郑州450000 [4]河南牧业经济学院,郑州450046 [5]上海市农业科学院农产品质量标准与检测技术研究所,上海201403

出　　处：《上海农业学报》2019年第1期71-75,共5页Acta Agriculturae Shanghai

基　　金：江苏省高校品牌专业建设工程资助项目(PPZY2015C230);河南牧业经济学院博士科研启动基金(5040229)

摘　　要：为研究蜂胶黄酮不同使用方法对豚鼠体液免疫和细胞免疫的影响,选取99只豚鼠用于试验研究,其中24只用于检验PPV疫苗的安全性,其余75只随机分成5组,分别为蜂胶黄酮佐剂PPV疫苗处理组(PFA)、油佐剂(OA) PPV疫苗+口服蜂胶黄酮处理组(OA-PFApo)、油佐剂(OA) PPV疫苗+肌注蜂胶黄酮处理组(OA-PFAim)、油佐剂PPV疫苗处理组(OA)和生理盐水对照组(CK)。结果表明:与油佐剂PPV疫苗组相比,蜂胶黄酮可以提高豚鼠细胞免疫和早期体液免疫; OA-PFAim组豚鼠体液免疫和细胞免疫均显著增强,而OA-PFApo组豚鼠早期体液免疫效果显著增强。综上,蜂胶黄酮可以直接作为一种PPV疫苗佐剂使用。The purpose of this study was to analyze the effect of propolis flavone in different applications on humoral immunity and cellular immunity in immunized guinea pigs.Ninety-nine guinea pigs were selected for this study,and 24 among them were used to determine the safety of PPV vaccine.Other 75 guinea pigs were randomly divided into proplis-flavone-adjuvanted PPV vaccine group(PFA),oil-adjuvanted PPV vaccine oral propolis flavone group(OA-PFApo),oil-adjuvanted PPV vaccine(OA)+propolis flavone by intramuscular injection group(OA-PFAim),oil-adjuvanted PPV vaccine(OA),and physiological salt solution control group.The results showed that,compared with OA group,propolis flavone adjuvant could enhance cellular immunity and early humoral immunity in immunized guinea pigs.In OA-PFAim group,cellular immunity and humoral immunity in guinea pigs were both significantly increased.In OA-PFApo group,early humoral immune was significantly enhanced.It’s concluded that propolis flavone can be used as adjuvant to prepare propolis flavone PPV vaccine.

关 键 词：蜂胶黄酮 猪细小病毒疫苗 体液免疫 细胞免疫 

分 类 号：S859.79[农业科学—临床兽医学]