植物源性食品中氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺残留的LC-MS/MS检测方法研究  

Determination method of fluopicolide,fenaminstrobin and chlorantraniliprole residues in plant foods by LC-MS/MS

作　　者：司文帅[1] 张颖[2] 张其才[1] 韩奕奕 杨晓君 白冰[1] 宋卫国[1] SI Wen-shuai;ZHANG Ying;ZHANG Qi-cai;HAN Yi-yi;YANG Xiao-jun;BAI Bing;SONG Wei-guo(Institute for Agro-Food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences, Shanghai 201403,China;Shanghai University of Medicine&Health Sciences,Shanghai 201318;Agri-products Quality and Safety Testing Center of Shanghai,Shanghai 201708,China)

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所,上海201403 [2]上海健康医学院,上海201318 [3]上海市农产品质量安全检测中心,上海201708

出　　处：《上海农业学报》2019年第1期76-79,共4页Acta Agriculturae Shanghai

基　　金：上海市农委科技兴农重点攻关项目[沪农科攻字(2015)第4-7号];上海市市级农口系统青年人才成长计划[沪农青字(2016)第1-25号];上海市科委长三角合作项目(16395810400)

摘　　要：采用液相色谱-串联质谱仪(LC-MS/MS)建立了植物源性食品中氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺残留的定量分析方法,样品经过乙腈涡旋提取后,无水MgSO4、PSA和C18分散固相萃取净化,LC-MS/MS检测。结果表明:在2—100μg/L的线性范围内,氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺的峰面积与其浓度呈良好线性关系,方法检出限为2μg/kg;在水稻、西瓜、青菜、番茄和黄瓜5种基质中,10μg/kg、20μg/kg和40μg/kg三个添加水平下,氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺回收率为88. 2%—107. 6%,相对标准偏差为1. 2%—8. 2%。该方法可以满足植物源性食品中氟吡菌胺、烯肟菌胺、氯虫苯甲酰胺残留检测的需要。The liquid chromatography-tandem mass spectrometry( LC-MS/MS) was developed to analyze fluopicolide,fenaminstrobin and chlorantraniliprole residues in plant foods. After extracted from sample with acetonitrile and cleaned up by dispersive solid-phase extraction with PSA and C18,fluopicolide,fenaminstrobin and chlorantraniliprole was determined by LC-MS/MS. As a result,The calibration curves showed good linearity over the concentration range of 2. 0-100 μg/L with correlation eoefficients above 0. 998,and the limits of determination( LOD) was 2. 0 μg/kg. The recoveries of fluopicolide,fenaminstrobin and chlorantraniliprole residues in plant foods were in the range of 88. 2% -107. 6% at three spiked levels( 10 μg/kg,20 μg/kg,40 μg/kg),and the relative standard deviations were 1. 2% -8. 2%.

关 键 词：植物源性食品 氟吡菌胺 烯肟菌胺 氯虫苯甲酰胺 液相色谱-串联质谱 

分 类 号：S481.5[农业科学—农药学] TS207.53[农业科学—植物保护]