响应面法优化橘核中3种柠檬苦素类成分的超声辅助提取工艺  

Optimization of the Ultrasonic-assisted Extraction Process of Three Limonoids Constituents in Citrus Seed by Response Surface Methodology

作　　者：郁林娜 程盛勇 付洋 鲁文琴 刘兴赋 罗喜荣 杨军[3] YU Lin-na;CHENG Sheng-yong;FU Yang;LU Wen-qin;LIU Xing-fu;LUO Xi-rong;YANG Jun(School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,Guizhou,China;Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou,China;Institute of Geochemistry,Chinese Academy of Sciences,Guiyang 550081,Guizhou,China)

机构地区：[1]贵州医科大学药学院,贵州贵阳550025 [2]贵州医科大学附属医院,贵州贵阳550004 [3]中国科学院地球化学研究所,贵州贵阳550081

出　　处：《食品研究与开发》2019年第5期133-137,共5页Food Research and Development

基　　金：贵州省科学技术基金项目(黔科合J字[2013]2037号);贵州省社发攻关项目(黔科合SY字[2015]3032号);贵州省联合基金项目(黔科合LH字[2014]7091号)

摘　　要：研究橘核中3种柠檬苦素类成分的超声提取辅助工艺。根据单因素试验结果,采用响应面法对提取工艺进行优化。结果显示优化提取工艺为甲醇作提取溶剂、提取时间40 min、提取温度33℃、液料比13∶1(mL/g),在此条件下柠檬苦素类成分总得率为11.804 5 mg/g,与模型预测值11.756 9 mg/g基本一致,该方法可用于提取橘核中的柠檬苦素、诺米林和黄柏酮。The optimum ustrasonic-assisted extraction technology of three limonoids constituents from citrus seed was investigated.According to the results of single factor test,the response surface method was used to optimize the extraction process.The optimum test conditions were as follow:the best solvent system was methanol,extraction time 40 min,temperature 33℃,and liquid-solid ratio 13∶1(mL/g).In verification test,the extraction yield of three limonoids constituents in citrus seed was 11.804 5 mg/g,basically consistent with the total rate of predicted value was 11.756 9 mg/g.The response surface method was feasible to optimize the extraction technology of limonin,nomilin and obacunone in citrus seed.

关 键 词：橘核 柠檬苦素 诺米林 黄柏酮 响应面法 

分 类 号：TQ464[化学工程—制药化工]