3D打印微小颗粒骨/聚乳酸-羟基乙酸共聚物支架材料的物理性能及其生物相容性  

Physical properties and biocompatibility of 3D printed bone microparticle/poly(lactic-co-glycolic acid) scaffold

作　　者：张敏波 彭齐峰 马亚萍 孔维军[1] 廖文波[1] Zhang Minbo;Peng Qifeng;Ma Yaping;Kong Weijun;Liao Wenbo(Department of Spinal Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China)

机构地区：[1]遵义医学院附属医院脊柱外科,贵州省遵义市563000

出　　处：《中国组织工程研究》2019年第14期2215-2222,共8页Chinese Journal of Tissue Engineering Research

基　　金：遵义市红花岗区科学技术项目[遵红科合社字(2016)09号];项目负责人:廖文波~~

摘　　要：背景:3D打印技术为制备具有高度个性化、精确调控孔隙率和孔径大小及孔径间连通率的理想骨组织工程支架提供了希望。目的:利用熔炉沉积型3D打印技术制备微小颗粒骨/聚乳酸-羟基乙酸共聚物复合可吸收支架,分析其物理性能和生物相容性。方法:利用熔炉沉积型3D打印技术制备微小颗粒骨/聚乳酸-羟基乙酸共聚物复合可吸收支架,检测支架的孔隙率、吸水率、水接触角及体外降解率。(1)细胞毒性实验:分别以正常培养基(阴性对照组)、复合可吸收支架浸提液(实验组)培养兔脂肪间充质干细胞,培养1,3,5,7 d,采用CCK-8法检测细胞增殖;(2)溶血实验:将兔抗凝血分别加入生理盐水、蒸馏水及复合可吸收支架浸提液中,50 min后检测溶血率;(3)急性毒性实验:将生理盐水、复合可吸收支架浸提液分别经腹腔注入兔体内,观察兔一般情况及重要脏器病理改变。结果与结论:①复合可吸收支架的孔隙率为(60.86±2.88)%,吸水率为(53.98±2.04)%,水接触角为(76.27±0.34)°;②体外浸泡于PBS中4周内,复合可吸收支架材料降解相对较慢,支架形态稳定,4周后降解速度加快,11周左右支架基本完全吸收,是一种相对理想的可吸收支架材料;③实验组与阴性对照组培养不同时间点的细胞增殖无差异(P> 0.05),复合可吸收支架的细胞毒性为1级;④复合可吸收支架的溶血率为3.8%,小于5%,符合生物医用材料溶血率要求;⑤注射可吸收支架浸提液72h内,兔未出现急性中毒反应表现,并且注射7 d后未见肝、心、肾等毒性病理改变;⑥结果表明,利用熔炉沉积型3D打印技术制备的微小颗粒骨/聚乳酸-羟基乙酸共聚物复合可吸收支架,具有良好的物理性能与生物相容性。BACKGROUND: 3D printing technology provides possibility for the preparation of an ideal bone tissue engineering scaffold with high individualization, precise regulation of the porosity, pore size and inter-aperture connectivity. OBJECTIVE: To study the physical properties and biocompatibility of allogeneic bone microparticle/poly(lactic-co-glycolic acid) composite absorbable scaffolds fabricated via fused deposition modeling 3D printing. METHODS: 3D printed bone microparticle/poly(lactic-co-glycolic acid) composite absorbable scaffolds were fabricated via fused deposition modeling. The porosity, water absorption, water contact angle and in vitro degradation rate of the scaffold were measured.(1) Cytotoxicity test: Rabbit adipose-derived mesenchymal stem cells were cultured in normal medium (negative control group) and composite scaffold extract (experimental group) for 1, 3, 5, 7 days, and cell counting kit-8 was then used to detect cell proliferation.(2) Hemolysis test: Rabbit anti-clotting was added to physiological saline, distilled water and composite scaffold extract, and the hemolysis rate was detected after 50 minutes.(3) Acute toxicity test: The physiological saline and composite scaffold extracts were intraperitoneally injected into the rabbits respectively to observe the general condition of rabbits and the pathological changes of important organs. RESULTS AND CONCLUSION:(1) The porosity, water absorption and water contact angle of the composite scaffold were (60.86±2.88)%,(53.98±2.04)% and (76.27±0.34)°, respectively.(2) After in vitro immersion in PBS for 4 weeks, the absorbable composite scaffold degraded relatively slowly, and the scaffold morphology remained stable, while the degradation rate accelerated after 4 weeks. The scaffold was almost completely absorbed at about 11 weeks, and it is a relatively ideal absorbable scaffold material.(3) There was no difference in cell proliferation between the experimental group and the negative control group at different time points (P > 0.05). The cytoto

关 键 词：3D打印 熔炉沉积型 骨组织工程 同种异体微小颗粒骨 聚乳酸-羟基乙酸共聚物 可吸收生物材料 可吸收支架 生物相容性材料 材料试验 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]