表面活性剂-超声协同提取红景天总黄酮工艺优选  

Process Optimization for Extraction of Total Flavonoids from Rhodiola Crenulata by Surfactant-Ultrasound Synergistic Extraction

作　　者：洪松彬[1] 何石兰[2] 黄海潮[3] 聂阳[3] HONG Songbin;HE Shilan;HUANG Haichao;NIE Yang(The First Affiliated Hospital of Guangzhou University of TCM,Guangzhou,Guangdong,China 510405;Ersha Island Branch Hospital,Guangdong Province Hospital of Traditional Chinese Medicine,Guangzhou,Guangdong,China 510105;Guangdong Food and Drug Vocational College, Guangzhou,Guangdong,China 510520)

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广东省中医院二沙岛分院,广东广州510105 [3]广东食品药品职业学院,广东广州510520

出　　处：《中国药业》2019年第4期8-11,共4页China Pharmaceuticals

基　　金：广东省医学科学技术研究基金项目[B2018184];广东省中医药局项目[20161035]

摘　　要：目的优选表面活性剂-超声协同提取红景天总黄酮的工艺。方法以总黄酮提取率为评价指标,对不同类型的表面活性剂进行筛选,再分别以表面活性剂体积分数、乙醇体积分数、超声功率、提取时间、料液比进行单因素试验,采用L_(27)(3~5)正交试验优选提取工艺。结果十二烷基硫酸钠(SDS)在60%乙醇中对总黄酮的增溶效果最好;正交试验优选的最佳提取工艺为以1.5%SDS作为增溶剂,超声功率为600 W,60%乙醇为溶剂,料液比为1∶15(g∶L),提取15 min。验证试验结果显示,总黄酮的平均提取率为4.04%,RSD为0.81%(n=5)。结论所建表面活性剂-超声协同提取工艺可经济、高效地提取红景天中总黄酮。Objective To optimize the synergistic extraction process of total flavonoids from Rhodiola crenulata by surfactant-ultrasound synergistic extraction.Methods With the extraction rate of total flavonoids as the evaluation index,different types of surfactant were screened.Single factor test was carried out with the volume fraction of surfactant,the volume fraction of ethanol,ultrasonic power,extraction time and solid-liquid ratio,respectively.The extraction conditions were optimized by L27(35)orthogonal test.Results The sodium lauryl sulfate(SDS)had the best solubilization effect on flavonoids in 60%ethanol.The optimum extraction process of orthogonal test was as follows:1.5%SDS was taken as solubilizer,ultrasonic power was 600 W,60%ethanol was taken as the solvent,the solid-liquid ratio was 1∶15(g∶L),the extraction time was 15 min.The verification result showed that the average extraction rate of total flavonoids was 4.04%and RSD was 0.81%(n=5).Conclusion The established surfactant-ultrasound synergistic extraction method is economical and efficient for extraction of total flavonoids from Rhodiola crenulata.

关 键 词：红景天 超声提取 表面活性剂 总黄酮 正交试验 工艺优化 

分 类 号：R932[医药卫生—生药学] R284.2[医药卫生—药学]