影响金黄色葡萄球菌杀白细胞素ED转录表达的培养条件  

Effects of culture conditions on transcriptional expression of leukocidin ED of Staphylococcus aureus

作　　者：杨涵 黄凯峰 徐溯 赵焕强[1] 徐晓刚 胡付品 何春燕[1] 龚芳[3] 刘庆中[1] YANG Han;HUANG Kaifeng;XU Su;ZHAO Huanqiang;XU Xiaogang;HU Fupin;HE Chunyan;GONG Fang;LIU Qingzhong(Department of Clinical Laboratory, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, China;Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai 200040, China;Department of Clinical Laboratory, The Third Hospital Affiliated to Nantong University, Nantong 226000, Jiangsu Province, China)

机构地区：[1]上海交通大学附属第一人民医院检验医学中心,上海200080 [2]复旦大学附属华山医院抗生素研究所,上海200040 [3]南通大学附属南通第三医院检验科,江苏南通226000

出　　处：《微生物与感染》2019年第1期23-29,共7页Journal of Microbes and Infections

基　　金：国家自然科学基金(81772247;81371872)

摘　　要：为探讨影响金黄色葡萄球菌杀白细胞素ED(leukocidin ED,LukED)转录表达的最佳培养条件,采用定量反转录-聚合酶链反应(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)检测金黄色葡萄球菌Newman菌株lukE在TSB、BHI、YCP、MHB和LB培养基中不同生长阶段(对数生长早期、中期、晚期和平台期)的表达情况。以表达量较低的培养基为基础,分别加入表达量最高的培养基的不同成分,分析促进lukED表达的物质。结果显示,lukE mRNA水平在YCP培养基中生长至对数晚期时表达最高;YCP组分酵母浸出物、酪蛋白氨基酸及丙酮酸钠均能显著促进lukE转录表达,前两者效果更好。This study aimed to explore the optimal culture conditions for transcriptional expression of leukocidin ED ( lukED ) of Staphylococcus aureus (S. aureus). The transcription level of lukE in S. aureus strain Newman was detected at different growth phases (early-exponential, middle-exponential, late-exponential and stationary phases) in TSB, BHI, YCP, MHB and LB media by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Based on the medium mediating the lowest transcription level of lukE , the different compositions of medium producing the highest mRNA level of lukE was added to discover the substances promoting lukED expression. The findings showed that lukE mRNA level was highest at the late-exponential phase in YCP medium, in which yeast extract, casamino acids and sodium pyruvate could all strikingly increase the expression of lukE , with the first two being better.

关 键 词：金黄色葡萄球菌 杀白细胞素ED MRNA水平 生长阶段 培养基 

分 类 号：R446.5[医药卫生—诊断学]