稀土配合物荧光探针的合成  

Synthesis of Lanthanide Complex-Based Luminescent Probes

作　　者：钱宇峰 李云辉[1] 曹雪 常宇[2] 刘宝峰[2] 潘利华[2] 田亚萍[3] QIAN Yu-feng;LI Yun-hui;CAO Xue;CHANG Yu;LIU Bao-feng;PAN Li-hua;TIAN Ya-ping(Department of Chemistry,Changchun University of Science and Technology ,Changchun 130022,China;Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun,130022,China;The First Hospital of Jilin University,Changchun 130022,China)

机构地区：[1]长春理工大学化学与环境工程学院,吉林长春130022 [2]中国科学院长春应用化学研究所,吉林长春130022 [3]吉林大学第一医院,吉林长春130022

出　　处：《标记免疫分析与临床》2019年第1期147-152,共6页Labeled Immunoassays and Clinical Medicine

基　　金：吉林省重点科技攻关项目(编号:20150204010YY);吉林省发改委项目(编号:2014Y115)

摘　　要：目的研制可用于时间分辨荧光免疫分析中的稀土配合物荧光探针。方法以6,6'-二溴二甲基-2,2'-联吡啶-4-甲酸甲酯和1,4,10,13-四氧-7,16-二氮环十八烷为反应原料,合成了Na^+-6,6-(二氮杂-18-冠醚-6)-二胺甲基-2,2'-联吡啶-4-甲酸甲酯,并通过核磁氢谱、质谱等分析手段确认了化合物的结构。结果对该化合物与Eu^(3+)连接形成的稀土配合物荧光探针的荧光性质进行研究,结果表明其激发光谱波长范围较宽,最大激发波长为363 nm,特征发射波长为583 nm与621 nm,归属于~5D_0-~7F_(j=1,2)跃迁,Stokes位移为258 nm,荧光寿命为1456μs。结论该稀土配合物荧光探针符合时间分辨荧光分析技术所需要求,具有广泛的应用前景。Objective To develop the lanthanide complex-based luminescent probes for the time-resolved fluorescence immunoassay. Methods The compound Na^+-6,6′-(diaza-18-crown-6)-bisaminomethyl}-2,2′-bipyridine-4-methyl formate was synthesized with the 6,6′-dibromo-dimethyl-2,2'-bipyridine-4-methyl formate and the 1,4,10,13-tetraoxa-7,16-diaza-cyclooctadecane.The structure was confirmed by proton nuclear magnetic resonance spectroscopy ( 1 H NMR)and mass spectrometry (MS). Results The luminescence spectroscopic properties of Eu(Ⅲ)complex-based luminescent probes were as follows: the excitation peak was at 363 nm,and the emission spectrum peaks were at 583 nm (^5 D0-^7F1 transition)and 621 nm (^5D0-^7F2 transition ).The Stokes shift was 258 nm and the luminescence lifetime was 1456 μ s. Conclusion The Eu(Ⅲ)complex-based luminescent probes developed in this study conform to the requirements of TRFIA,with an widely applicable prospect.

关 键 词：时间分辨荧光免疫分析 稀土配合物 荧光探针 

分 类 号：R446.6[医药卫生—诊断学]