1976年中国唐山地震生存截瘫患者淋巴细胞亚群水平检测研究  

Detection of Lymphocyte Subsets in Patientswith Paraplegia after the 1976 Tangshan Earthquake in China

作　　者：王菊惠[1] 袁宝军[1] 李超[1] 裴斐[2] 李佳强 杨宏秀[1] WANG Ju-hui;YUAN Bao-jun;LI Chao;PEI Fei;LI Jia-qiang;YANG Hong-xiu(Department of Laboratory,Kailuan General Hospital,Tangshan,Hebei 063000;Department of Neurosurgery,Kailuan General Hospital,Tangshan,Hebei,063000;Department ofClinical Laborat ory,Tangshan Paraplegia Sanatorium,Hebei Tangshan 063003,China)

机构地区：[1]开滦总医院检验科,河北唐山063000 [2]开滦总医院神经外科,河北唐山063000 [3]唐山市截瘫疗养院检验科,河北唐山063003

出　　处：《现代检验医学杂志》2019年第1期38-40,46,共4页Journal of Modern Laboratory Medicine

摘　　要：目的测定1976年中国唐山地震生存截瘫患者外周血淋巴细胞亚群水平,评估截瘫患者40多年后细胞免疫功能的状况。方法采集110例截瘫患者静脉血,用流式细胞术测定患者T淋巴细胞亚群(包括CD3^+,CD4^+Th细胞,CD8^+Ts细胞,CD4^+/CD8^+),CD19^+B淋巴细胞和CD16^+CD56^+NK细胞,并与正常对照作比较。同时,按性别分层比较各项目的差异及其之间的相关性。结果截瘫组与对照组比较,截瘫组CD8^+T细胞升高,CD4^+/CD8^+和CD16^+CD56^+NK细胞水平下降,差异均有统计学意义(F=1.375,1.052,0.674,均P<0.05)。CD3^+,CD4^+T细胞和CD19^+B细胞差异无统计学意义(F=0.800,1.490,0.223,均P>0.05)。按性别分层,CD19^+B细胞女性组高于男性组,两组结果差异具有统计学意义(F=0.038,均P<0.05),其他指标无明显差异(F=0.002～1.808,均P>0.05)。CD4^+与CD8^+,CD4^+与CD4^+/CD8^+,CD4^+与CD16^+CD56^+,CD8^+与CD4^+/CD8^+,CD8^+与CD16^+CD56^+,CD19^+与CD16^+CD56^+均存在显著相关性(P<0.01)。结论唐山地震截瘫患者细胞免疫功能严重紊乱。Objective The peripheral blood lymphocyte subsets of paraplegic patients after the 1976 Tangshan earthquake in China were measured and the immune function of paraplegic patients after 40 years was evaluated.Methods Venous blood samples were collected from 110 paraplegic patients.T lymphocyte subsets(including CD3^+,CD4^+Th cells and CD8^+Ts cells),CD19^+B lymphocyte and CD16^+CD56^+NK cells were measured by flow cytometry and compared with those of normal controls.The differences among items and their correlations were compared by gender stratification.Results Compared with the control group,in paraplegic group the levels of CD8^+T cells increased,the levels of CD4^+/CD8^+and CD16^+CD56^+NK cells decreased.The difference was statistically significant(F=1.375,1.052,0.674,all P<0.05).There was no significant difference in the number of CD3^+,CD4^+,CD19^+B cells(F=0.800,1.490,0.223,all P >0.05).The sex stratified CD19^+B cells were higher in the female group than in the male group,and the result difference was statistically significant(F=0.038,P<0.05),but there was no significant change in other indexes(F=0.002~1.808,all P >0.05).CD4^+and CD8^+,CD4^+and CD4^+/CD8^+,CD4^+and CD16^+CD56^+,CD8^+and CD4^+/CD8^+,CD8^+and CD16^+CD56^+,CD19^+and CD16^+CD56^+were significantly correlated(P<0.01).Conclusion Patients with paraplegia after the Tangshan earthquake have severe cellular immune dysfunction.

关 键 词：截瘫患者 细胞免疫功能 T淋巴细胞亚群 B淋巴细胞 NK细胞 

分 类 号：R64[医药卫生—外科学] R392.11[医药卫生—临床医学]