隔药灸对克罗恩病大鼠结肠NLRP3炎症小体及IL-1?调节作用的实验研究  

Experimental Study on the Regulating Effect of Herbal Cake-partitioned Moxibustion on NLRP3 Inflammasome and IL-1β in Colons of Crohn's Disease Rats

作　　者：张霁 吴丽洁[1] 李志元 张丹[3] 智方圆 杨延婷[1,3] 吴丹艳 赵越 李茜莹 马晓芃 ZHANG Ji;WU Li-jie;LI Zhi-yuan;ZHANG Dan;ZHI Fang-yuan;YANG Yan-ting;WU Dan-yan;ZHAO Yue;LI Xi-ying;MA Xiao-peng(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Zhejiang Provincial Hosptial of TCM,Hangzhou 310006,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,China)

机构地区：[1]上海中医药大学,上海201203 [2]浙江省中医院,杭州310006 [3]上海市针灸经络研究所,上海200030

出　　处：《上海针灸杂志》2019年第2期119-126,共8页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家重点基础研究发展计划(2015CB554501);国家自然科学基金项目(81674073);上海市卫生计生系统优秀学科带头人培养计划项目(2017BR047)

摘　　要：目的通过观察隔药灸对克罗恩病大鼠结肠NLRP3炎症小体(NLRP3、ASC、Caspase-1)及下游炎症因子IL-1?表达的影响,探讨隔药灸治疗克罗恩病的抗炎机制。方法将清洁级雄性SD大鼠随机分为正常组、模型组、隔药灸组和假灸组4组。采用三硝基苯磺酸(2,4,6-trinitrobenzenesulfonic acid, TNBS)与乙醇混合溶液灌肠制备大鼠克罗恩病模型。造模成功后,隔药灸组取天枢(双)、气海穴进行隔药饼灸治疗;假灸组取穴、操作同隔药灸组,但不点燃艾炷。治疗结束后,记录各组大鼠结肠长度及CMDI评分;采用HE染色,光学显微镜下观察各组大鼠结肠组织形态结构,并应用免疫组织化学技术检测各组大鼠结肠NLRP3、ASC、Caspase-1及IL-1?的表达。结果与正常组比较,模型组大鼠结肠组织损伤严重,表现为裂隙样溃疡,炎症细胞浸润伴水肿,部分大鼠结肠黏膜可见肉芽组织形成,且结肠NLRP3、ASC、Caspase-1、IL-1?蛋白表达显著增加(均P<0.05);与模型组比较,隔药灸组大鼠结肠损伤有所修复,炎症反应减轻,结肠NLRP3、ASC、Caspase-1及IL-1?蛋白表达显著降低(均P<0.05);假灸组大鼠结肠损伤程度、炎症反应与模型组类似,两组结肠NLRP3、ASC、Caspase-1、IL-1?表达比较,差异均无统计学意义(P>0.05)。结论隔药灸能下调克罗恩病大鼠结肠NLRP3、ASC、Caspase-1及IL-1?的蛋白表达,促进结肠炎性损伤的修复。Objective To explore the action mechanism of moxibustion in treating Crohn's disease(CD)by observing the effect of herbal cake-partitioned moxibustion on the expression of NLRP3 inflammasome(NLRP3,ASC and Caspase-1)and IL-1β.Method Male Sprague Dawley(SD)rats of clean conventional grade were randomized into a normal control group(NG),a model control group(MG),a herbal cake-partitioned moxibustion control group(MoxG),and a sham moxibustion control group(SMG).The CD rat models were developed by using the mixture of 2,4,6-trinitrobenzene sulfonic acid(TNBS)and alcohol via enema.When the models were successfully developed,the MoxG received moxibustion intervention at bilateral Tianshu(ST25)and Qihai(CV6),and the SMG were administered by unlighted moxa cones.At the end of treatment,the length of each rats'colon and the colon macroscopic damage index(CMDI)score were recorded,the histopathological variations of rats'colons were observed by adopting HE staining and light microscope,and the expressions of NLRP3,ASC,Caspase-1 and IL-1βin rats'colons were determined by using immunohistochemical technique.Result Compared to the NG,the MG had its rats'colons present with severe damages,fissured ulcers and inflammatory cell infiltration with edema,and granulomas in submucosa of some colons,and its expressions of NLRP3,ASC,Caspase-1 and IL-1βincreased significantly(P<0.05);compared to the MG,the MoxG had rats'colons present with improved structures and reduced intestinal inflammation,and its expressions of NLRP3,ASC,Caspase-1 and IL-1βdropped significantly(P<0.05);the SMG had its rats'colon inflammation present similarly to the MG,and its expressions of NLRP3,ASC,Caspase-1 and IL-1βhad no significant difference(P >0.05).Conclusion Herbal Cake-partitioned moxibustion can down-regulate the expressions of NLRP3,ASC,caspase-1 and IL-1βin CD rats'colons to promote the repair of colon damage.

关 键 词：药饼灸疗法 克罗恩病 NLRP3炎症小体 间接灸 大鼠 

分 类 号：R2-03[医药卫生—中医学]