丹参注射液对糖尿病大鼠肾小管TLR4蛋白表达的影响  

Effects of Danshen Injection on TLR4 protein expression in the renal tubular of diabetic rats

作　　者：徐仲儒 阮旭 杨晔 尹登科 XU Zhong-ru;RUAN Xu;YANG Ye;YIN Deng-ke(School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Institute for Pharmaceuticcal Sciences,Anhui Provincial Acade.my of Chinese Medicine,Hefei 230012,China;Anhui Provincial Key Laboratory for Chinese Herbal Compound,Hefei 230012,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽省中医药科学院药物制剂研究所,安徽合肥230012 [3]中药复方安徽省重点实验室,安徽合肥230012

出　　处：《中成药》2019年第2期279-284,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81102682);安徽省自然科学基金(1708085MH216);安徽省高校自然科学基金(KJ2017A290)

摘　　要：目的研究丹参注射液对糖尿病大鼠肾小管Toll样受体4 (TLR4)蛋白表达的影响。方法高糖高脂饲料联合腹腔注射链脲佐菌素建立大鼠糖尿病模型,40只大鼠随机分为正常组、模型组、二甲双胍组、丹参注射液组,每组10只。给药6周后,HE染色观察大鼠肾组织病理变化,免疫组化染色观察TLR4蛋白表达,Western blot检测高糖环境下大鼠肾小管上皮细胞(NRK-52E)增殖及TLR4蛋白表达。结果丹参注射液缓解了大鼠肾小管病变。与模型组比较,丹参注射液组TLR4蛋白表达显著降低(P<0. 01);高糖环境下其质量浓度为30 mg/m L时可显著促进NRK-52E细胞增殖(P<0. 05),并呈剂量依赖性地显著降低TLR4蛋白表达(P<0. 05,P<0. 01)。结论丹参注射液可保护糖尿病大鼠肾小管,降低高糖环境下NRK-52E细胞TLR4蛋白表达,从而改善糖尿病肾病。AIM To study the effects of Danshen Injection on Toll-like receptor 4(TLR4)protein expression in the renal tubular of diabetic rats.METHODS Forty rats were randomly divided into normal group,model group,metformin group and Danshen Injection group,10 rats in each group,after which diabetic rat models were established by high-glucose and high-fat diet combined with intraperitoneal injection of streptozotocin.HE staining and immunohistochemistry staining succeeded by 6-week corresponding administration were applied to observing pathological changes and TLR4 protein expression of rat renal tissue,Western blot was used to detect the proliferation and TLR4 protein expression of rat renal tubular epithelial cells(NRK-52E)under high-glucose environment.RESULTS Danshen Injection alleviated rat renal tubular lesions.Compared with the model group,the Danshen Injection group demonstrated markedly decreased TLR4 protein expression(P<0.01),at whose concentration of30 mg/m L,significant promotion of NRK-52 E cell proliferation under high-glucose environment was achieved,as well as the obvious dose-dependent TLR4 protein expression decrease(P<0.05,P<0.01).CONCLUSION Danshen Injection improves diabetic nephropathy due to its protection for the renal tubular of diabetic rats and its power in decreasing the TLR4 protein expression of NRK-52 E cells under high-glucose environment.

关 键 词：丹参注射液 糖尿病 肾小管 Toll样受体4(TLR4) 

分 类 号：R285.5[医药卫生—中药学]