不同产地拉萨大黄HPLC指纹图谱  

HPLC fingerprints of Rheum lhasaense from different growing areas

作　　者：徐晨阳 郭明里 方芳 李宁 龚云麒 XU Chen-yang;GUO Ming-li;FANG Fang;LI Ning;GONG Yun-qi(Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;KPC Pharmaceuticals,Inc,Kunming 650100,China)

机构地区：[1]云南中医学院,云南昆明650500 [2]昆药集团股份有限公司,云南昆明650100

出　　处：《中成药》2019年第2期358-363,共6页Chinese Traditional Patent Medicine

基　　金：云南省科技计划项目(2013BC001)

摘　　要：目的建立不同产地拉萨大黄Rheum lhasaense A. J. Li et P. K. Hsiao HPLC指纹图谱。方法拉萨大黄75%甲醇提取物的分析采用Grace Apollo C18柱(250 mm×4. 6 mm,5μm);流动相乙腈-水(含0. 1%磷酸),梯度洗脱;体积流量1. 0 m L/min;柱温20℃;检测波长319 nm。结果 20批样品HPLC指纹图谱中有9个共有峰,相似度均在0. 90以上。曲札茋苷、去氧土大黄苷的平均含有量分别为4. 36%、2. 68%。结论该方法稳定可靠,可用于拉萨大黄药材的质量控制。AIM To establish the HPLC fingerprints of Rheum lhasaense A.J.Li et P.K.Hsiao from different growing areas.METHODS The analysis of 75%methanol extract of R.lhasaense was developed on a 20℃thermostatic Grace Apollo C18column(250 mm×4.6 mm,5μm),with the mobile phase comprising of acetonitrilewater(containing 0.1%phosphoric acid)flowing at 1.0 m L/min in a gradient elution manner,and the detection wavelength was set at 319 nm.RESULTS There were nine common peaks in the fingerprints of twenty batches of samples,with the similarities of more than 0.90.Average content of quzhazhigan and deoxyrhaponticin of twenty batches of samples were 4.36%,2.68%,respectively.CONCLUSION This stable and reliable method can be used for the quality control of R.lhasaense.

关 键 词：拉萨大黄 HPLC指纹图谱 曲札茋苷 去氧土大黄苷 

分 类 号：R284.1[医药卫生—中药学]