苓甘五味姜辛汤对脂多糖诱导的急性肺损伤大鼠免疫细胞和炎性细胞因子的影响  

Effects of Linggan Wuwei Jiangxin Decoction on Immune Cells and Inflammatory Cytokines in Rats with Acute Lung Injury Induced by Lipopolysaccharide

作　　者：王宝娟[1] 崔利锋[1] 李冬霞[1] 张云虹[1] 杨伟锋[1] 马国安[2] Wang Baojuan;Cui Lifeng;Li Dongxia(The Second People′s Hospital of Hengshui City,Hebei,Hengshui 053000,China)

机构地区：[1]河北省衡水市第二人民医院,河北衡水053000 [2]河北省衡水市哈励逊国际和平医院,河北衡水053000

出　　处：《中国中医急症》2019年第2期220-224,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：河北省衡水市科学技术研究与发展计划项(15023)

摘　　要：目的观察苓甘五味姜辛汤对脂多糖(LPS)诱导的急性肺损伤(ALI)大鼠的可能作用并探讨其机制。方法将90只清洁级的SD大鼠按随机数字表法分为6组(空白对照组,ALI模型组,地塞米松组与中药高、中、低剂量组),每组15只,连续用药27 d后采用LPS暴露式气管滴注方法制备大鼠ALI模型,实验结束前30 min收集腹主动脉血并进行左侧支气管肺泡灌洗以收集灌洗液,取大鼠右肺下叶组织,进行苏木精-伊红(HE)染色后显微镜下观察肺组织病理形态学改变并进行病理学评分;取大鼠右肺中叶置电子天平称量肺组织湿质量,然后将组织置于70℃烘箱48 h至恒重后再次称量干质量,计算肺湿/干质量比(W/D)值。采用流式细胞技术对大鼠动脉血中T淋巴细胞亚群进行测定;将支气管肺泡灌洗液采用ELISA法测定白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)的浓度。结果病理形态学方面,ALI模型组大鼠肺组织结构出现破坏,肺泡水肿明显,肺泡腔内炎症细胞浸润,肺间质弥漫性充血水肿;地塞米松组及中药高、中剂量组的肺组织上述变化较ALI模型组明显减轻。病理学评分方面,ALI模型组评分明显高于空白对照组(P <0.05);经不同药物干预后,地塞米松组和中药高、中剂量组评分均明显降低(P <0.05)。W/D比值方面,与空白对照组相比,ALI模型组肺组织W/D比值明显增加(P <0.05);地塞米松组与中药高剂量组、中剂量组肺组织W/D比值较ALI模型组明显减轻(P <0.05)。免疫细胞方面,ALI模型组动脉血中CD3+、CD4+、和CD4+/CD8+水平较空白对照组明显降低(P <0.05),CD8+水平明显升高(P <0.05)。与ALI模型组比较,中药高剂量组、中剂量组动脉血中CD3+、CD4+和CD4+/CD8+水平明显升高(P <0.05),CD8+水平明显降低(P <0.05);地塞米松组和中药低剂量组与ALI模型组相比各项指标变化不明显(P> 0.05)。LPS造模后大鼠BALFObjective:To investigate the possible effects and mechanism of Linggan Wuwei Jiangxin Decoction on acute lung injury(ALI)rats induced by lipopolysaccharide(LPS).Methods:90 clean SD rats were randomly divided into 6 groups(the blank group,ALI group,dexamethasone group and Linggan Wuwei Jiangxin Decoction high,medium and low dose groups),15 rats in each.The pathological morphology changes of the lung was observed under the microscope after HE staining and the pathological were scored.The wet/dry ratio(W/D)of the lung tissue was calculated.T lymphocyte subsets in rat arterial blood were determined by flow cytometry.The concentrations of IL-1β,IL-6,IL-8 and TNF-αin bronchoalveolar lavage fluid were determined by ELISA.Results:1)In pathological morphology,the lung tissue structure of ALI group was damaged;alveolar edema was obvious,inflammatory cells infiltrated in alveolar cavity,diffuse congestion and edema in pulmonary interstitium.The above changes of dexamethasone group and high and medium dose groups were significantly alleviated compared with those of model group.2)Pathological scores of ALI group were significantly higher than those of the blank group(P<0.05).After intervention with different drugs,scores of dexamethasone group and high and medium dose groups were significantly lower(P<0.05).3)Compared with the blank group,the W/D ratio in ALI group increased significantly(P<0.05),while that in dexamethasone group,high-dose group and medium-dose groups was significantly lower than that in ALI group(P<0.05).4)In terms of immune cells,the levels of CD3+,CD4+,and CD4+/CD8+in ALI group were significantly lower than those of the blank group(P<0.05),and the levels of CD8+were significantly higher(P<0.05).Compared with ALI group,the levels of CD3+,CD4+,and CD4+/CD8+in high-dose group and middle-dose group were significantly higher(P<0.05),while the levels of CD8+were significantly lower(P<0.05).The indexes of dexamethasone group and low-dose group were not significantly changed compared with ALI group(P >0.05).5)Th

关 键 词：急性肺损伤 苓甘五味姜辛汤 脂多糖 T淋巴细胞亚群 白细胞介素类 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]