北京地区荷斯坦母牛群体HH1遗传缺陷基因抽样调研  

The Random Sampling for Genetic Defect HH1 in Chinese Holstein Cows in Beijing Area

作　　者：吕小青[1] 劳兰兰 赵凤[1] 李艳华[1] 麻柱[1] 刘林[1] LV Xiao-qing;LAO Lan-lan;ZHAO Feng;LI Yan-hua;MA Zhu;LIU Lin(Beijing Dairy Cattle Center,Beijing 100192;College of Animal Science and Technology,China Agricultural University,Beijing 100193)

机构地区：[1]北京奶牛中心,北京100192 [2]中国农业大学动物科技学院,北京100193

出　　处：《中国奶牛》2019年第1期12-14,共3页China Dairy Cattle

基　　金：现代奶牛产业技术体系北京市创新团队岗位专家;优良奶牛育种评估体系建设与选育技术研究(D171100002417001)

摘　　要：HH1(holstein haplotype1)是近年来在荷斯坦牛群中发现的一种遗传缺陷,其致病机理是牛5号染色体APAF1(apoptotic peptidase activating factor 1)基因上的C/T突变,导致编码谷氨酰胺的密码子突变为终止密码子,纯合时个体致死,大多数表现为妊娠早期流产。本研究运用竞争性等位基因特异性PCR(KASP)方法,共随机检测了北京地区158头荷斯坦母牛,发现HH1携带者20头,携带率为12.66%,提示HH1在我国荷斯坦母牛群体中存在一定的比例,牛场有必要定期对HH1携带者进行筛查并合理选种选配。HH1 is a recessively inherited disorder that was recently identified in Holstein cattle, the embryo with homozygous defective alleles will die during early pregnancy. The molecular mechanism of HH1 has been identified as a C/T mutation in APAF1 gene on chromosome 5 that leads to a codon mutation encoding glutamine as a termination codon. In this study, competitive allele-specific PCR(KASP) method was used to detect the frequency of genetic defect gene (HH1) in 158 Chinese Holstein cows. As a result, 20 HH1 carriers were identified and carrying rate was 12.66%, indicating that the HH1 has a certain proportion in Chinese Holstein population, we need to detect the carriers of HH1 and avoid the risk mating in dairy cattle breeding work.

关 键 词：荷斯坦母牛 遗传缺陷 HH1 APAF1基因 

分 类 号：S823.3[农业科学—畜牧学]