不同培养基对春小麦×玉米单倍体胚分化成苗的影响  

Effects of Different Media on Haploid Plantlets Production from the Spring Wheat × Maize Cross

作　　者：王重[1,2] 樊哲儒 张跃强[1,2] 李剑峰 高新[1,2] 王子霞 WANG Zhong;FAN Zhe-ru;ZHANG Yue-qiang;LI Jian-feng;GAO Xin;WANG Zi-xia(Research Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Key Laboratory of Crop Ecophysiology and Farming System in Desert Oasis Region,Ministry of Agriculture,P.R. China,Urumqi 830091,China)

机构地区：[1]新疆农业科学院核技术生物技术研究所,乌鲁木齐830091 [2]农业部荒漠绿洲作物生理生态与耕作重点实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2018年第11期1988-1993,共6页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区青年科学基金项目(2013211B23);新疆农业科学院青年科学基金项目(xjnkq-2013025)~~

摘　　要：【目的】筛选适宜春小麦单倍体胚分化成苗的培养基,获得稳定且高效的单倍体胚培养方法。【方法】分别使用8种不同配方培养基对春小麦单倍体胚进行离体培养,使其分化成苗,根据成苗率及生长情况筛选培养基。【结果】在不添加激素的1/2 MS培养基中春小麦单倍体胚成苗率最高(73.49%),在1/2MS+0.1 mg/L 2,4-D培养基中春小麦单倍体胚能够一步成苗,且生长健壮,根系发达。【结论】1/2 MS培养基较B5培养基更适宜春小麦单倍体胚的分化成苗,2,4-D对于春小麦单倍体胚的根系发育具有明显的促进作用,2,4-D>IAA,1/2 MS+0.1 mg/L 2,4-D培养基是春小麦单倍体胚分化成苗的适宜培养基。【Objective】This project aims to screen suitable medium for differentiation of spring wheat haploid embryos and obtain a stable and efficient method.【Method】The haploid embryos were differentiated into plantlets using 8 different mediums,and the mediums was screened according to the seedling rate and growth conditions.【Result】The seedling rate of spring wheat haploid embryos was the highest(73.49%)in 1/2 MS medium without hormones.The haploid embryos were induced one-step-seedling in 1/2 MS+0.1 mg/L 2,4-D medium,with stronger plantlets and their roots developed well.【Conclusion】The 1/2 MS medium was more suitable for the differentiation of spring wheat haploid embryos than the B5 medium,and the 2,4-D had more significant effect on the development of roots and differentiation of embryos.The medium of 1/2 MS+0.1 mg/L 2,4-D was a suitable medium for haploid embryos differentiation of spring wheat.

关 键 词：春小麦 单倍体胚 分化 培养基 

分 类 号：S512[农业科学—作物学]