髓鞘发育关键调控因子MYRF多克隆抗体的制备与鉴定  

Preparation and Verification of Polyclonal Antibodies of Myelin Development Key Factor MYRF

作　　者：杜君卿 易敏 杨爱芬 黄浩[1] DU Junqing;YI Min;YANG Aifen;HUANG Hao(School of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 310036,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2019年第1期41-45,65,共6页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31771621);浙江省自然科学基金青年基金项目(Q16C090017)

摘　　要：MYRF是近年发现的中枢神经系统少突胶质细胞髓鞘化的关键转录因子,但是有效的抗MYRF抗体的缺乏限制了与MYRF相关的髓鞘发育和疾病的研究.通过原核表达和纯化His标签融合的MYRF抗原多肽片段并免疫新西兰兔,制备获得了anti-MYRF多抗.Western Blot、组织和细胞中的免疫荧光等实验进一步确认了该抗体可以特异性地识别小鼠MYRF蛋白.MYRF抗体的成功制备将有助于MYRF相关的髓鞘发育研究.MYRF is a critical factor of myelin development in the central nervous system(CNS)identified in recent years.However,the lack of efficient anti-MYRF antibodies limits the studies on MYRF-related myelin development and diseases.A method for preparing anti-MYRF polyclonal antibodies by immunizing New Zealand rabbits with bacterial His-tagged MYRF peptides is reported.The specificity and efficiency of anti-MYRF antibodies are verified by Western Blot and immunofluorescence in vitro and in vivo.The successful preparation of anti-MYRF antibodies will benefit the researches on MYRF-related myelin development.

关 键 词：MYRF 多克隆抗体 少突胶质细胞 髓鞘 

分 类 号：Q189[生物学—神经生物学]