桡骨骨折模型兔骨折愈合:特异性环氧化酶2抑制剂与非特异性环氧化酶抑制剂的比较  

Efficacy of specific versus non-specific cyclooxygenase-2 inhibitors in the healing of rabbit models of tibial fractures

作　　者：陈海涛 安玉光 Chen Haitao;An Yuguang(Department of Surgery,People’s Hospital of Yongning District,Nanning 530299,Guangxi Zhuang Autonomous Region,China)

机构地区：[1]南宁市邕宁区人民医院外科,广西壮族自治区南宁市530299

出　　处：《中国组织工程研究》2019年第15期2385-2390,共6页Chinese Journal of Tissue Engineering Research

基　　金：南宁市科学研究与技术开发项目(20153087);项目负责人:陈海涛~~

摘　　要：背景:非特异性环氧化酶抑制剂和特异性环氧化酶抑制剂都是常用的镇痛、消炎类非类固醇类药,两种药物治疗对骨折创伤愈合的效果及用药时间的比较缺乏相关实验依据。目的:对比非特异性环氧化酶2抑制剂与特异性环氧化酶2抑制剂对兔桡骨骨折愈合的效果。方法:3-5月龄新西兰大白兔36只,雌雄不限,实验动物由广西医科大学实验动物中心提供。建立兔单侧桡骨骨折(3 mm)模型共36只,随机分为3组:模型组予以等剂量蒸馏水灌胃;特异性环氧化酶2抑制剂组予以1.15 mg/(kg·d)依托考昔灌胃;非特异性环氧化酶抑制剂组予以7.6 mg/(kg·d)阿司匹林灌胃,每组12只。在术后2,4,8周对各组兔桡骨组织进行大体观察和组织病理切片染色观察,检测兔血清骨钙素及骨组织转化生长因子β1、血管内皮生长因子水平。结果与结论:①术后观察发现非特异性环氧化酶抑制剂组造模侧桡骨组织在骨痂的形成改建及愈合情况上均要优其他2组;②术后的各个时间点非特异性环氧化酶抑制剂组血清骨钙素水平明显高于特异性环氧化酶2抑制剂组(P <0.05);③苏木精-伊红染色结果:非特异性环氧化酶抑制剂组在胶原纤维、软骨组织、骨小梁及骨基质的形成时期均早于特异性环氧化酶2抑制剂组;④术后第4周非特异性环氧化酶抑制剂组转化生长因子β1和血管内皮生长因子免疫组织化学平均吸光度值均明显大于特异性环氧化酶2抑制剂组(P <0.05);⑤结果说明,特异性环氧化酶2抑制剂修复兔桡骨骨折的时间相对非特异性环氧化酶更长,说明环氧化酶2特异性抑制剂对骨折愈合的效率低于非特异性环氧化酶抑制剂。BACKGROUND:Non-specific cyclooxygenase inhibitor and specific cyclooxygenase inhibitor are commonly used nonsteroidal anti-inflammatory and analgesic drugs,but there is a lack of experimental evidence concerning the effect of these drugs on the fracture healing and the administration time.OBJECTIVE:To investigate the effect of non-specific cyclooxygenase-2 inhibitor versus specific cyclooxygenase-2 inhibitor on the healing of tibial fractures in rabbits.METHODS:Thirty-six 3-5-month-old New Zealand rabbits either in sexes were selected(provided by Laboratory Animal Center of Guangxi Medical University).Rabbit models of unilateral tibial fractures(3 mm)were established and randomly divided into modeling group(same volume of distilled water via gavage),specific cyclooxygenase-2 inhibitor group(1.15 mg/kg.d etoricoxib via gavage),and non-specific cyclooxygenase-2 inhibitor group(7.6 mg/kg.d aspirin via gavage),with 12 rabbits in each group.At 2,4 and 8 weeks postoperatively,the fracture healing was observed and assessed by gross observation and histopathological sections.The serum level of osteocalcin,and levels of transforming growth factor 兝1 and vascular endothelial growth factor were detected.RESULTS AND CONCLUSION:(1)The non-specific cyclooxygenase-2 inhibitor group was superior to the other two groups in the formation and remodeling of callus and healing.(2)The serum level of osteocalcin in the non-specific cyclooxygenase-2 inhibitor group was significantly higher than that in the specific cyclooxygenase-2 inhibitor group(P<0.05).(3)Hematoxylin-eosin staining results:the non-specific cyclooxygenase-2 inhibitor group was earlier than the specific cyclooxygenase-2 inhibitor group in the formation of collagen fibers,cartilage tissue,trabecular bone and bone matrix.(4)The average absorbance value of transforming growth factor and vascular endothelial growth factor in the non-specific cyclooxygenase-2 inhibitor group was significantly higher than those in the specific cyclooxygenase-2 inhibitor group at 4 weeks po

关 键 词：特异性环氧化酶2抑制剂 非特异性环氧化酶抑制剂 骨折 转化生长因子β1 血管内皮生长因子 环氧化酶2 组织工程 

分 类 号：R446[医药卫生—诊断学]