黄连素对大鼠肾脏缺血再灌注损伤的影响及相关炎症因子的表达变化  

Effect of berberine on renal ischemia reperfusion injury and changes of expression of related inflammatory factors in rats

作　　者：郑海雅[1] 兰俊[2] 吕雷立[1] 黄振强 ZHENG Haiya;LAN Jun;LV Leili;HUANG Zhenqiang(Clinical Laboratory,Lishui People’s Hospital in Zhejiang Province,Lishui 323000,China;Department of Orthopaedics,Lishui People’s Hospital in Zhejiang Province,Lishui 323000,China)

机构地区：[1]浙江省丽水市人民医院检验科,浙江丽水323000 [2]浙江省丽水市人民医院骨科,浙江丽水323000

出　　处：《中国现代医生》2019年第2期32-36,169,共6页China Modern Doctor

基　　金：浙江省中医药科学研究基金项目(2015ZA224);浙江省医药卫生科技计划(2014KYB316)

摘　　要：目的探讨黄连素在大鼠肾脏缺血再灌注损伤中的作用。方法 40只雄性SD大鼠随机分为对照组(S组)、模型组(IR组)、黄连素组(H组)和黄连素+Exendin-(9-39)组(HE组)。S组给予生理盐水及行假手术处理,IR组给予生理盐水,H组给予黄连素,HE组给予黄连素+Exendin-(9-39),三组制作大鼠肾脏缺血再灌注模型。观察肾组织病理学改变,检测造模前及造模12 h和24 h后血清中肌酐(SCr)、尿素氮(BUN)水平及血清白细胞介素(IL)-10、血管内皮生长因子(VEGF)以及肾组织中肿瘤坏死因子(TNF)-α、IL-1β、IL-6的含量。本次实验研究时间为2016年9～11月。结果模型制作成功,血清BUN、Cr水平在H组中低于IR组(P<0.05),而HE组低于H组(P<0.05);IR组、H组、HE组中IL-10、IL-6、TNF-α、VEGF水平均高于S组(P<0.05),HE组IL-6、TNF-α水平较H组明显降低(P<0.05)。四组IL-1β水平无明显变化。结论黄连素能有效改善肾脏缺血再灌注大鼠的肾功能,其机制可能与抑制肾脏组织炎症反应有关。Objective To investigate the role of berberine in renal ischemia-reperfusion injury in rats.Methods 40 male Sprague-Dawley rats were randomly divided into control group(group S),model group(group IR),berberine group(group H)and berberine+Exendin-(9-39)group(group HE).Group S was given saline and sham surgery;group IR was given saline,group H was given berberine,and group HE was given berberine+Exendin-(9-39).Rat kidney ischemia-reperfusion models were made in the three groups.The pathological changes in renal tissues were observed.Serum creatinine(SCr),urea nitrogen(BUN)levels,and serum interleukin(IL)-10,vascular endothelial growth factor(VEGF),tumor necrosis factor in kidney tissues,as well as the contents of(TNF)-α,IL-1β,and IL-6 were measured before modeling and 12 h and 24 h after modeling.The study time of his experiment was from September 2016 to November 2016.Results The model was successfully established.Serum BUN and Cr levels were lower in the group H than in the group IR(P<0.05).The group HE was lower than the group H(P<0.05);the levels of IL-10,IL-6,TNF-αand VEGF in group IR,group H and group HE were all higher than those in group S(P<0.05).The levels of IL-6 and TNF-αin the group HE were significantly lower than those in the group H(P<0.05).There were no significant changes in the levels of IL-1βin the four groups.Conclusion Berberine can effectively improve the renal function of the rats receiving renal ischemia-reperfusion.Its mechanism may be related to the inhibition of inflammatory reactions in the kidney tissues.

关 键 词：黄连素 肾脏缺血再灌注 炎症因子 保护作用 

分 类 号：R692[医药卫生—泌尿科学]