Study on Rapid Detection of Tetracyclines,Fluoroquinolones and Sulfonamides in Milk  

作　　者：Yuping WAN Xiaosheng WU Tingting CUI Dongshan CAO Zhaoqin WANG Linchen WANG Chun-yan YANG Fangyang HE 

机构地区：[1]Beijing Kwinbon Biotechnology Co.,Ltd. [2]Beijing Engineering Research Center of Food Safety Immunodetection [3]Beijing Wanger Biotechnology Co.,Ltd.

出　　处：《Agricultural Biotechnology》2019年第1期241-245,共5页农业生物技术（英文版）

基　　金：Supported by Hebei Science and Technology Program(16275507D)

摘　　要：Aiming at the market demand for rapid detection of tetracyclines,fluoroquinolones and sulfonamides in milk,a golloidal gold immunochromatography test strip for simultaneous detection of tetracyclines,fluoroquinolones and sulfonamides in milk was prepared based on the principle of competitive inhibition immunochromatography. The performance indicators of the test strip were verified. The results showed that the test strip can simultaneously detect 4 tetracyclines,13 fluoroquinolones and 13 sulfonamides,and the detection limits all can meet the national residue limits; the tests strip exhibited false positive rate≤5% and false negative rate = 0; and no cross-reaction with other drugs was commonly found in milk,indicating good specificity. The method is simple,rapid,and has low cost and easy popularization. It provides a means for realizing on-site rapid detection and is of important practical significance to guarantee of safety of milk and dairy products in China.Aiming at the market demand for rapid detection of tetracyclines,fluoroquinolones and sulfonamides in milk,a golloidal gold immunochromatography test strip for simultaneous detection of tetracyclines,fluoroquinolones and sulfonamides in milk was prepared based on the principle of competitive inhibition immunochromatography. The performance indicators of the test strip were verified. The results showed that the test strip can simultaneously detect 4 tetracyclines,13 fluoroquinolones and 13 sulfonamides,and the detection limits all can meet the national residue limits; the tests strip exhibited false positive rate≤5% and false negative rate = 0; and no cross-reaction with other drugs was commonly found in milk,indicating good specificity. The method is simple,rapid,and has low cost and easy popularization. It provides a means for realizing on-site rapid detection and is of important practical significance to guarantee of safety of milk and dairy products in China.

关 键 词：MILK TETRACYCLINES FLUOROQUINOLONES SULFONAMIDES COLLOIDAL gold IMMUNOCHROMATOGRAPHY assay 

分 类 号：S[农业科学]