一株南海指海绵共附生真菌多样性及其抗菌活性  

Diversity and antibacterial activities of fungi associated with the South China Sea sponge

作　　者：杜晓娜 高渊皓 张雁 徐炜[3] 骆祝华[3] DU Xiao-Na;GAO Yuan-Hao;ZHANG Yan;XU Wei;LUO Zhu-Hua(Sanquan College of Xinxiang Medical University,Xinxiang,Henan 453000,China;Key Laboratory of Marine Biogenetic Resource,Third Institute of Oceanography,State Oceanic Administration,Xiamen,Fujian 361005,China;College of Life Science and Technology,Jinan University,Guangzhou,Guangdong 510632,China;Xinxiang Hawthorn Utilization Engineering Center,Xinxiang,Henan 453000,China)

机构地区：[1]新乡医学院三全学院,河南新乡453000 [2]新乡市山楂综合利用工程技术研究中心,河南新乡453000 [3]国家海洋局第三海洋研究所国家海洋局海洋生物遗传资源重点实验室,福建厦门361005 [4]暨南大学生命科学技术学院,广东广州510632

出　　处：《菌物学报》2019年第1期64-73,共10页Mycosystema

基　　金：福建省自然科学基金"东山珊瑚自然保护区珊瑚共附生真菌群落生态分布及其生物活性潜力评估"(26185006);国家海洋局第三海洋研究所基本科研业务费专项资助项目(海三科20160339)~~

摘　　要：本研究通过采用6种不同的培养基对南海指海绵上的共附生真菌进行平板涂布法分离培养,结合ITS-rDNA基因序列特征和形态学特征进行鉴定来研究海绵共附生真菌多样性。共分离培养出55株(21种)真菌菌株,优势菌属为木霉菌属Trichoderma sp.(31株),其中的哈茨木霉Trichoderma harzianum 22株作为优势菌种,占真菌菌株总数的40%。其余真菌则属于枝孢菌属Cladosporium、迈耶氏酵母属Meyerozyma、茎点霉属Phoma、红酵母属Rhodotorula、青霉菌属Penicillium、曲霉属Aspergillus等10个属,还有两株未鉴定到种的子囊菌。选取金黄色葡萄球菌Staphylococcus aureus等7种病原指示菌,采用琼脂扩散法对21种真菌的代表性菌株进行抗菌活性筛选,其中有3种真菌至少对一种指示菌表现出抑菌效果,占菌株种类总数的11%。Six different kinds of culture media are used to isolate and cultivate the fungi associated with the South China Sea sponge. The isolates obtained totaled 55, belonging to 22 species. The identification wasmade through morphological observation in combination with ITS-rDNA blast homology analysis. The dominant genus is Trichoderma(31 isolates), and Trichoderma harzianum is dominant species(22 isolates), accounting for 40% of the total number of isolates. The remainder belongs to Cladosporium, Meyerozyma, Rhodotorula, Microsphaeropsis, Penicillium, Aspergillus, Phoma, Candida, Pestalotiopsis, and Engyodontium. Besides, two ascomycete isolates are unidentified. Seven indicator bacteria including Staphylococcus aureus were selected for antibacterial activity test. By using agar diffusion method, the bacteriostatic effect of the fungal isolates was verified. The result demonstrated that three isolates showed antibacterial activity for at least an indicator bacteria.

关 键 词：南海指海绵 共生真菌 ITS-rDNA的扩增 抗菌菌株 

分 类 号：Q939.9[生物学—微生物学]