胶陀螺光敏毒性成分  

Photosensitive toxic components of Bulgaria inquinans

作　　者：包海鹰[1,2] 李志军[1,2] 杨树东[2] 马伟才 白璐[1] 王新斌[1] 图力古尔[2] 李玉[2] BAO Hai-Ying;LI Zhi-Jun;YANG Shu-Dong;MA Wei-Cai;BAI Lu;WANG Xin-Bin;BAU Tolgor;LI Yu(Key Laboratory of Medicinal Fungal Resources and Development and Utilization,Jilin Agricultural University,Changchun,Jilin 130118,China;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun,Jilin 130118,China)

机构地区：[1]吉林农业大学药用菌物资源及其开发利用重点研究室,吉林长春130118 [2]吉林农业大学教育部食药用菌工程研究中心,吉林长春130118

出　　处：《菌物学报》2019年第1期117-126,共10页Mycosystema

基　　金：长江学者和创新团队发展计划项目(IRT-15R25);吉林省高等学校高端科技创新平台建设(#2014B-1)~~

摘　　要：食用未经碱处理过的胶陀螺子实体,会出现嘴巴肿胀、暴露在日光下的皮肤灼痛等过敏症状,一直以来困扰着长白山当地居民对这一美食的需求。本文旨在找到胶陀螺Bulgaria inquinans中光敏活性成分。将胶陀螺子实体粉末用丙酮回流提取,浓缩得到浸膏,加吐温-80溶解后灌胃昆明种小鼠,进行光敏活性试验。把出现光敏反应的小鼠处死后,取下红肿的小鼠耳朵用甲醇索式提取得活性组分,进行薄层色谱层析和柱层析,得到一种活性单体化合物,经核磁共振和电子轰击质谱波谱综合解析鉴定该化合物为邻苯二甲酸二异丁酯(DiBP)。结果表明DiBP就是引起胶陀螺光敏中毒反应的活性成分,且经碱水降解后毒性降低。The photosensitive toxic symptom caused by Bulgaria inquinans puzzled local residents of Changbai mountainous area all along. Eating the fungus without alkali treatment can cause swelling of mouth and burning pains of skin when exposure under sunlight. The study aimed at finding the photosensitive toxic components of B. inquinans. The active extract was obtained from B. inquinans fruiting bodies, using as dissolvent acetone. The extract was administrated to mice, and then the active component was further obtained from the methanolextract of mice’s red swelling ears. The active compound was identified as diisobutyl phthalate(DiBP) by using 1 H-NMR and 13 C-NMR, and EI-MS spectroscopy. DiB P was proved to be the photosensitive toxic compound of B. inquinans causing photosensitivity of mice. DiBP can be degraded in alkaline water and the toxicity can be decreased.

关 键 词：胶陀螺 光敏毒性 单体化合物 邻苯二甲酸二异丁酯 

分 类 号：R284[医药卫生—中药学]