大秃马勃培养基配方的优化  

Optimization of Medium Formula for Mycelia Growth of Calvatia gigantean

作　　者：史鑫 王宏杰 王鸿磊[1] 丁强[1] 王红艳[1] SHI Xin;WANG Hongjie;WANG Honglei;DING Qiang;WANG Hongyan(Yantai Research Institute,China Agricultural University,Yantai,Shandong 264670,China)

机构地区：[1]中国农业大学烟台研究院,山东烟台264670

出　　处：《贵州农业科学》2019年第1期79-82,共4页Guizhou Agricultural Sciences

基　　金：山东省农业重大应用技术创新课题"双孢菇优良品种高效栽培及病虫害防控技术研究与应用";山东省农业科学院科技创新重点项目"作物秸秆;畜禽废弃物栽培食用菌及有机肥制造关键技术研究"(2014CXZ02-2);山东省农业科学院项目"山东省产业体系食用菌烟台综合试验站"(SDAIT-07-14)

摘　　要：为大秃马勃工厂化生产提供技术支撑,采用单因素试验与Box-Behnken响应面分析法相结合,对大秃马勃菌丝培养基配方进行优化。结果表明:大秃马勃菌丝最适碳源和氮源分别为淀粉和豆饼粉,最优培养基配方为7.10g/L豆饼粉+14.11g/L淀粉+6.00g/L土豆浸粉+2.0g/L KH2PO4+0.5g/LMgSO4+20g/L琼脂水。该条件下大秃马勃菌丝生长速率达3.45mm/d,长满平板(9cm)的时间缩短至11d,菌丝生长量达3.795cm。The medium formula for mycelia growth of Calvatia gigantean was optimized by the single-factor experiment and Box-Behnken response surface analysis to provide the technical reference for industrialized production of C.gigantean.Result:The optimum carbon and nitrogen source for mycelia growth of C.gigantean is starch and soybean cake powder respectively.The optimum medium formula for mycelia growth of C.gigantean includes 7.10 g/L soybean cake powder+14.11 g/L starch+6.00 g/L potato powder+2.0 g/L KH 2PO 4+0.5 g/L MgSO 4+20 g/L agar.The growth rate,growth days to cover the petri plate(9 cm)and growth amount of C.gigantean mycelia under the optimum medium formula are 3.45 mm/d,11 d and 3.795 cm respectively.

关 键 词：大秃马勃 药用真菌 菌丝生长 Box-Behnken响应面分析法 

分 类 号：S646[农业科学—蔬菜学]