MSTN敲除对肌肉增强因子2(MEF2C)和miR-222表达调控的影响  

Regulation Effect of MSTN Knockout on Expression of Myocyte Enhancer Factor Gene 2 (MEF2C) and miR-222

作　　者：肖伟 蔡刚志 华再东[2] 任红艳[2] 朱喆 肖红卫[2] 郑新民[2] 顾玉芳[1] 毕延震[2] 杨玉莹[1] XIAO Wei;CAI Gangzhi;HUA Zaidong;REN Hongyan;ZHU Zhe;XIAO Hongwei;ZHENG Xinmin;GU Yufang;BI Yanzhen;YANG Yuying(College of Animal Science,Yangtze University,Jingzhou,Hubei 434025;Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding,Institute of Animal Husbandry and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064;Key Laboratory of Animal Genetics,Breeding and Reproduction in Plateau Mountainous Region,Ministry of Education,Guiyang,Guizhou 550025;Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction,Guiyang,Guizhou 550025;College of Animal Science,Guizhou University,Guiyang,Guizhou 550025,China)

机构地区：[1]长江大学动物科学学院,湖北荆州434025 [2]湖北省农业科学院畜牧兽医研究所动物胚胎工程及分子育种湖北省重点实验室,湖北武汉430064 [3]高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳550025 [4]贵州省动物遗传育种与繁殖重点实验室,贵州贵阳550025 [5]贵州大学动物科学学院,贵州贵阳550025

出　　处：《贵州农业科学》2019年第1期83-86,89,共5页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"猪MSTN通过MEF2C/miR222/SCD5特有分子通路调控脂肪酸代谢的机理"(31772577);湖北省自然科学基金项目"零风险基因编辑家畜育种技术研究";"杰出青年"(2018CFA043);湖北省农业科技创新中心项目"家畜高效繁育技术研究"(2018-620-004-001);湖北省农业科学院领军人才培养计划项目"猪基因编辑育种技术研究"

摘　　要：为脂肪沉积转录调控通路的研究奠定基础,通过生物信息学、Western印迹和RT-qPCR等方法研究肌肉抑制素(MSTN)敲除后对潜在调控脂肪沉积的肌肉增强因子2(MEF2C)和miR-222的表达调控作用。结果表明,在miR-222启动子区域预测到MEF2C的结合位点,Western印迹显示肌肉抑制素敲除后MEF2C表达量上调,qPCR结果表明当MEF2C高表达后miR-222表达量上调3.48倍(P<0.01)。说明MSTN敲除后引起MEF2C高表达,进而促使miR-222表达量上升。The regulation effect of MSTN knockout on expression of myocyte enhancer factor gene 2(MEF2C)and miR-222 was studied by using bioinformatics,Western blot and RT-qPCR methods to lay the foundation for research of fat deposition transcriptional regulation pathways.Result:The binding site of MEF2C can be predicted in miR-222 promoter region.The Western blot shows that MEF2C expression is up-regulation after MSTN knockout.The qPCR results show that miR-222 expression increases by 3.48 times(P<0.01)after high expression of MEF2C,which indicates that the high expression of MEF2C caused by MSTN knockout can promote rise of miR-222 expression.

关 键 词：肌肉抑制素 肌肉增强因子2 MIR-222 生物信息学 脂肪沉积 

分 类 号：S813.3[农业科学—畜牧学]