厚朴叶过氧化物酶对水体中壬基酚的去除效果  

Scavenging Nonylphenol Efficiency of Peroxidase Extracted from Magnolia officinalis Leaves

作　　者：王雨雪 赵广华 张小萌 唐密 吴水涵 肖强 WANG Yuxue;ZHAO Guanghua;ZHANG Xiaomeng;TANG Mi;WU Shuihan;XIAO Qiang(Hubei University for Nationalities,Hubei key Laboratory of Biological Resources Protection and Utilization,Enshi,Hubei 445000;School of Forestry and Horticulture,Hubei University for Nationalities,Enshi,Hubei 445000,China)

机构地区：[1]湖北民族学院生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族学院林学园艺学院,湖北恩施445000

出　　处：《贵州农业科学》2019年第1期116-121,共6页Guizhou Agricultural Sciences

基　　金：国家自然科学基金项目"一氧化氮调控植物硒代谢机制研究"(31260057);"珍稀濒危植物珙桐种子萌发特性及其种质长期保存研究"(31460203);湖北省大学生创新创业训练计划项目"厚朴叶过氧化物酶清除内分泌干扰物NP的研究"(201710517038)

摘　　要：为提高厚朴叶片资源的综合利用价值,采用双水相萃取和硫酸铵沉淀方法提取厚朴叶过氧化物酶,研究其与H2O2形成的催化体系降解壬基酚的效果和反应条件。结果表明:经过双水相萃取和硫酸铵沉淀后的厚朴叶过氧化物酶部分纯化酶纯化倍数为8.4倍,比活力为25 663.1 U/mg Pro,回收率为68.6%;当温度为20～50℃,pH 3～7,H2O2与壬基酚摩尔浓度比为5～30,厚朴过氧化物酶浓度为60U/mL时,对10μmol/L壬基酚的去除率最高,达99.9%。反应体系中腐植酸浓度>5mg/L时,厚朴过氧化物酶对壬基酚的去除率下降10%左右,具有用于类激素类污染物去除的较好潜力。The peroxidase was extracted from Magnolia officinalis leaves by two aqueous phase extraction and ammonium sulfate precipitation methods and the degrading nonylphenol efficiency and reaction condition of the catalytic system formed by the peroxidase and H 2O 2 were studied to improve comprehensive utilization value of M.officinalis leaf resources.Result:The purification fold,specific activity and recovery of the part purified peroxidase after extraction of two aqueous phase extraction and ammonium sulfate precipitation are 8.4,25 663.1 U/mg Pro and 68.6%respectively.The scavenging 10μmol/L nonylphenol rate of the peroxidase extracted from M.officinalis leaves can reach 99.9%when temperature,pH,molar concentration ratio between H 2O 2 and nonylphenol and peroxidase concentration are 20-50℃,3-7,5-30 and 60 U/mL respectively.The scavenging nonylphenol rate of the peroxidase extracted from M.officinalis leaves decreases by 10%when the humic acid concentration in the reaction system is >5 mg/L,which indicates that the peroxidase is a better potential of scavenging hormone-like pollutants.

关 键 词：厚朴 叶 过氧化物酶 壬基酚 污染处理 

分 类 号：S567.11[农业科学—中草药栽培] X52[农业科学—作物学]