红景天苷上调HIF-1α减轻高糖诱导的大鼠肾小球内皮细胞损伤  

Salidroside attenuates high glucose-induced rat renal glomerular endothelial cell injury via up-regulating HIF-1α expression

作　　者：谢瑞燕 方雪玲 Hamze I.RAGE 崔彤霞[1] 朱伟平[1] XIE Rui-yan;FANG Xue-ling;Hamze I.RAGE;CUI Tong-xia;ZHU Wei-ping(Department of Nephrology,The Fifth Affiliated Hospital of Sun Yat-sen University,Zhuhai 519000,China)

机构地区：[1]中山大学附属第五医院肾内科,广东珠海519000

出　　处：《中国病理生理杂志》2019年第2期237-242,共6页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金博士启动项目(No.2016A030310193)

摘　　要：目的:观察体外不同浓度葡萄糖对大鼠肾小球内皮细胞(rRGECs)表达低氧诱导因子1α(HIF-1α)、血管内皮生长因子A(VEGFA)及血管内皮钙黏素(VE-cadherin)的影响,探讨红景天苷减轻高糖诱导rRGECs损伤的作用及可能相关机制。方法:体外培养rRGECs,分为正常糖组、高糖(20、30和50 mmol/L)组、高渗组及红景天苷+高糖组。采用MTT法检测rRGECs的活力;RT-qPCR法检测rRGECs内HIF-1α、VEGFA及VE-cadherin的mRNA表达;Western blot法检测rRGECs内HIF-1α蛋白的表达。结果:与正常糖组相比,培养24 h后,高糖(20mmol/L)组rRGECs HIF-1α的mRNA及蛋白表达均上调(P <0.05);培养120 h后,高糖组HIF-1αmRNA表达下调(P <0.05)。与正常糖组相比,培养24 h和120 h后,高糖组rRGECs内VE-cadherin的mRNA表达下调(P <0.05)。与正常糖组相比,培养24 h后,高糖组rRGECs内VEGFA的mRNA表达上调(P <0.05);培养120 h后,高糖组rRGECs内VEGFA的mRNA表达下调(P <0.05)。与正常糖组相比,培养24 h和120 h后,高渗组rRGECs内HIF-1α、VE-cadherin及VEGFA的mRNA表达无变化。与高糖组相比,培养24 h后,红景天苷(50μmol/L)组rRGECs的活力增加(P <0.01),且细胞内HIF-1α和VE-cadherin的mRNA及HIF-1α蛋白表达均上调(P <0.05)。结论:体外高糖培养能影响rRGECs表达HIF-1α,可能与细胞活力、葡萄糖的浓度、作用时间及HIF/VEGF通路有关。红景天苷能减轻高糖诱导的rRGECs损伤,其机制可能与增加rRGECs内HIF-1α的表达有关。AIM:To observe the expression of hypoxia-inducible factor-1α(HIF-1α),vascular endothelial growth factor A(VEGFA)and vascular endothelial cadherin(VE-cadherin)in cultured rat renal glomerular endothelial cells(rRGECs)exposed to glucose at different concentrations in vitro,and to verify the hypothesis that salidroside atte-nuates high glucose(HG)-induced injury of rRGECs by boosting HIF-1α level.METHODS:rRGECs were divided into 4 group:normal glucose(NG)group,HG groups,hypertonic group and salidroside+HG group.The viability of rRGECs was measured by MTT assay.The mRNA expression of VEGFA,VE-cadherin and HIF-1α was detected by RT-qPCR.The protein expression of HIF-1α was determined by Western blot.RESULTS:Compared with NG group,the mRNA and protein expression of HIF-1α was increased when the rRGECs were treated with glucose at concentration of 20 mmol/L for 24 h(P<0.01).Compared with NG group,the mRNA expression of HIF-1α was decreased in HG groups for 120 h(P<0.05).Compared with NG group,the mRNA expression of VE-cadherin was significantly down-regulated in HG groups for 24 h or 120 h(P<0.05).Compared with NG group,the mRNA expression of VEGFA was increased in HG groups at 24 h(P<0.05),while the mRNA expression of VEGFA was decreased at 120 h(P<0.05).Compared with NG group,no statistical difference in the mRNA expression levels of HIF-1α,VE-cadherin and VEGFA in DM group was observed.Compared with HG group,salidroside promoted the viability of rRGECs(P<0.01),and up-regulated the mRNA expression of HIF-1α and VE-cadherin,and the protein expression of HIF-1α(P<0.05).CONCLUSION:High glucose regulates HIF-1α expression in rRGECs in connection with cell viability,the concentration of glucose,the culture time and HIF/VEGF signaling.Salidroside promotes rRGEC growth against high glucose-induced cell apoptosis via up-regulating the expression of HIF-1α.

关 键 词：糖尿病肾病 低氧诱导因子1Α 肾小球内皮细胞 红景天苷 

分 类 号：R587.2[医药卫生—内分泌] R363.2[医药卫生—内科学]