荚果蕨贯众总黄酮的定性定量研究  

Determination and thin layer identification of Matteuccia struthiopteris Todaro

作　　者：孟庆庆 特布新 白明刚 吴香杰[2] 布和巴特尔[2] 奥.乌力吉 MENG Qingqing;TE Buxin;BAI Minggang;WU Xiangjie;Buhebateer;Ao·WuLiji(Inner Mongolia Research Institute Of traditional Mongolian medicine engineering technology,Tongliao 028000,China;Inner Mongolia University for Nationalities,Tongliao 028000,China)

机构地区：[1]内蒙古蒙医药工程技术研究院,内蒙古通辽028000 [2]内蒙古民族大学蒙医药学院,内蒙古通辽028000

出　　处：《中国民族民间医药》2018年第24期33-36,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:建立比色法测定荚果蕨贯众总黄酮的含量方法,薄层鉴别方法。方法:采用比色法测定荚果蕨贯众总黄酮的含量,薄层色谱鉴别法。结果:回归方程为y=0.4703x-0.0204,r=0.9998,样品浓度在0.2～1.2 mg/25mL范围内,与其吸光度呈良好线性关系;平均回收率为98.05%(RSD=0.66%)。薄层色谱的斑点Rf值适宜,显色清晰,色谱分离好。结论:该方法简便、快捷、准确、重复性好。Objective To establish a method for the determination of total flavonoids in Matteuccia struthiopteris Todaro by colorimetric method.Methods The content of total flavonoids in Matteuccia struthiopteris Todaro were determined by colorimetric method,and the method was identified by thin layer chromatography.Results The regression equation was y=0.4703 x-0.0204,r=0.9998,The sample concentration was in the range of 0.2 mg/25 mL^1.2 mg/25 mL,with a good linear relationship with the absorbance.The average recovery was 98.05%(RSD=0.66%).Thin layer chromatography spots Rf value is appropriate,clear color,good chromatographic separation.Conclusion The method is simple,rapid,accurate and reproducible.

关 键 词：荚果蕨贯众 比色法 含量测定 薄层鉴别 

分 类 号：R284.1[医药卫生—中药学]