消痰化瘀中药对非酒精性脂肪肝大鼠脂质代谢和相关基因表达的影响  

Effects of Chinese Medicine for Dispersing Phlegm and Removing Blood Stasis on Lipid Metabolism and Related Gene Expression in Rats with Nonalcoholic Fatty Liver

作　　者：刘宇[1] 郭建利[2] 刘晨旭 韩雪[1] 石铖[1] 路帅[2] 郝蕾[1] 张一昕[1] LIU Yu;GUO Jian-li;LIU Chen-xu;HAN Xue;SHI Cheng;LU Shuai;HAO Lei;ZHANG Yi-xin

机构地区：[1]河北中医学院,石家庄050200 [2]石家庄医学高等专科学校,石家庄050599

出　　处：《河北中医药学报》2019年第1期1-5,共5页Journal of Hebei Traditional Chinese Medicine and Pharmacology

基　　金：河北省高等学校科学技术研究项目:No.QN2016107;河北中医药管理局资助项目:No.2015077;河北中医学院青年教师科研基金项目:No.QNZ2015005

摘　　要：目的:研究消痰化瘀中药对非酒精性脂肪肝(NAFLD)大鼠脂质代谢和肝脏相关基因[过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)和磷酸烯醇式丙酮酸激酶(PEPCK) mRNA]表达的的影响,探讨其治疗非酒精性脂肪肝的作用机制。方法:将60只SD大鼠随机分正常组、造型组(模型组)、东宝肝泰(阳性药)组以及消痰化瘀中药高、中、低剂量组,除正常组外各组通过高脂饲料喂养复制NAFLD大鼠模型,并给予相应药物治疗,8周后观察各组大鼠血清总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、血糖(FBG)、胰岛素(FINS)和肝组织中TC、TG的含量改变,观察肝组织PGC-1α和PEPCK mRNA的表达变化以及肝组织病理形态学改变。结果:消瘀化痰中药能降低模型大鼠TC、TG、FFA、ALT、AST、FBG、FINS的含量或活性,促进PGC-1α和PEPCK mRNA的表达,改善胰岛素抵抗(IR)和肝组织的脂变程度。结论:消痰化瘀中药之所以具有治疗NAFLD的作用,可能与其能够调控PGC-1α和PEPCK mRNA表达,抑制糖异生、减少肝糖输出,改善体内胰岛素抵抗水平,纠正脂类物质代谢的紊乱有关。Objective:To study the effects of Chinese medicine for dispersing phlegm and removing blood stasis on lipid metabolism and expression of liver-related genes,peroxisome proliferator-activated receptor-γcoactivator 1α(PGC-1α)and mRNA of phosphoenolpyruvate kinase(PEPCK)in rats with nonalcoholic fatty liver disease(NAFLD),and to explore its mechanism of action in treating nonalcoholic Fatty liver.Methods:Sixty SD rats were randomly divided into normal group,model group,Dongbao Gantai(positive drug)group and high,middle and low dose groups of Chinese medicine.Except the normal group,each group was fed with high fat diet to replicate the model of NAFLD rats and given corresponding drug treatment.After 8 weeks,the serum total cholesterol(TC),triglyceride(TG),free fatty acid(FFA),alanine aminotransferase(ALT),glutamic oxaloacetic aminotransferase(AST),blood sugar(FBG),insulin(FINS)and the changes of TC and TG contents in liver tissues were observed.The expressions of PGC-1αand PEPCK mRNA and the pathological changes of liver tissues were observed.Results:The Chinese medicine could reduce the content or activity of TC,TG,FFA,ALT,AST,FBG,FINS,promote the expression of PGC-1αand PEPCK mRNA,and improve the insulin resistance(IR)and the degree of hepatic lipid degeneration in model rats.Conclusion:The Chinese herbal medicine for eliminating phlegm and removing blood stasis has the effect of treating NAFLD,which may be related to its ability to regulate the expression of PGC-1αand PEPCK mRNA,inhibit gluconeogenesis,reduce hepatic glycogen output,improve insulin resistance level in vivo,and correct the disorder of lipid metabolism.

关 键 词：消瘀化痰中药 非酒精性脂肪肝 痞满 积聚 脂质代谢 PGC-1α PEPCK 基因 

分 类 号：R285.5[医药卫生—中药学]