Psammaplin A对牛老化卵母细胞体外发育的影响  

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

作　　者：王华清 高青山[1,2] 姜文杰 赵予晗[1,2] 金庆国 李英花 许永男 WANG Huaqing;GAO Qingshan;JIANG Wenjie;ZHAO Yuhan;JIN Qingguo;LI Yinghua;XU Yongnan(Yanbian Yellow Cattle Germplasm Resources Conservation Engineering Research Center, Yanji 133000,China;Yanbian University Agricultural College, Yanji 133000,China)

机构地区：[1]吉林省延边黄牛种质资源保护工程研究中心,延吉133000 [2]延边大学农学院,延吉133000

出　　处：《中国畜牧兽医》2019年第2期497-503,共7页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省教育厅"十三五"科学技术研究;吉教科合字[2016]第256号;延大科合字(2015)第20号

摘　　要：为探讨组蛋白去乙酰化抑制剂Psammaplin A(PsA)对牛老化卵母细胞体外发育的影响,本试验将卵母细胞随机分为对照组、老化组和50mmol/L PsA处理的老化组(PsA组)。应用免疫荧光染色和JC-1检测牛卵母细胞孤雌激活后的囊胚率、囊胚中的细胞数、细胞凋亡、活性氧(reactive oxygen species,ROS)、谷胱甘肽(glutathione,GSH)和胚胎线粒体膜电位强度。结果显示,老化组囊胚率显著低于PsA组及对照组(P<0.05),PsA组囊胚率与对照组间无显著差异(P>0.05)。对照组及PsA组囊胚内细胞数显著高于老化组(P<0.05),PsA组囊胚内细胞数与对照组间无显著差异(P>0.05);老化组细胞凋亡率显著高于对照组及PsA组(P<0.05),PsA组细胞凋亡率显著高于对照组(P<0.05)。老化组MⅡ期卵母细胞的GSH水平显著低于对照组与PsA组(P<0.05),对照组与PsA组的GSH水平无显著差异(P>0.05)。老化组1细胞期胚胎ROS水平显著高于对照组和PsA组(P<0.05),PsA组ROS水平显著高于对照组(P<0.05)。老化组4-8细胞早期胚胎的线粒体膜电位强度显著低于对照组和PsA组(P<0.05),对照组线粒体膜电位强度显著高于PsA组(P<0.05)。综上所述,PsA可有效延缓牛卵母细胞的老化并提高卵母细胞和胚胎质量。To investigate the effect of histone deacetylation inhibitor Psammaplin A(PsA)on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group(PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species(ROS),glutathione(GSH)and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups(P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group(P >0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group(P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group(P >0.05).The apoptosisrate in aging group was significantly higher than that of control and PsA groups(P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group(P<0.05).The GSH level of MⅡoocytes in aging group was significantly lower than that of control and PsA groups(P<0.05).There was no significant difference in GSH level between control and PsA groups(P >0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups(P<0.05).The ROS level in PsA group was significantly higher than that of control group(P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups(P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group(P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.

关 键 词：牛卵母细胞 Psammaplin A(PsA) 活性氧 线粒体膜电位 

分 类 号：S823[农业科学—畜牧学]