兔脾脏成纤维型细胞RS-17系的建立及其生物学特性  

Establishment and biological characteristics of rabbit spleen fibroblast cell line RS-17

作　　者：罗怡琳[1] 蒙正群 周丽军[1] 王印[1,3] 姚学萍[1] 杨泽晓[1] 罗燕[1] 耿毅[1] LUO Yi-lin;MENG Zheng-qun;ZHOU Li-jun;WANG Yin;YAO Xue-ping;YANG Ze-xiao;LUO Yan;GENG Yi(College of Veterinary Medicine, Sichuan Agricultural University,Wenjiang 611130, China;Guizhou Qin Bang Food Safety Science and Technology Co. Ltd., Guiyang 550000, China;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Wenjiang 611130, China)

机构地区：[1]四川农业大学动物医学院,四川温江611130 [2]贵州勤邦食品安全科学技术有限公司,贵州贵阳550000 [3]动物疫病与人类健康四川省重点实验室,四川温江611130

出　　处：《江苏农业学报》2019年第1期130-135,共6页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31402222);四川省科技支撑计划项目(2016N20002)

摘　　要：采用胰酶消化法培养技术,对乳兔脾脏组织的细胞进行原代培养,从脾脏组织中分离出1株成纤维型细胞,命名为RS-17。通过对细胞传代培养条件优化,细胞标志性蛋白检测,细胞生长曲线分析,细胞核型以及细胞致瘤性等测定,结果显示,RS-17细胞具有血清依懒性,成纤维细胞标志性蛋白检测阳性,具有正常的核型,无致瘤性,且目前细胞传至95代仍可稳定生长。这为RHDV(兔出血症病毒)感染机制等相关研究提供了细胞材料。The cells of the spleen tissue of the rabbit were primary cultured by trypsin digestion technique,and a fibroblast-like cell was isolated from the spleen tissue and named as RS-17.The subculture conditions was optimized,cellular marker proteins were detected by immunofluorescence,and the growth curve was analyzed,karyotype and tumorigenicity of the RS-17 were detected.The results showed that the RS-17 was fibroblast cell and had the normal karyotype,normal growth characteristic,no tumorigenicity.The cell has been passaged stably to the 95 generations.These results provide cellular material for studies related to the infection mechanism of rabbit hemorrhagic disease virus(RHDV).

关 键 词：乳兔 脾脏组织 细胞系 生物学特性 

分 类 号：S829.1[农业科学—畜牧学]