磷脂脂肪酸(PLFA)法检测内蒙古沙化梁地不同坡位羊柴(Hedysarum laeve Maxim)根围土壤微生物群落结构  

Characteristics of soil microbial community structure in the rhizosphere of Hedysarum laeve Maxim revealed by phospholipid fatty acid (PLFA)

作　　者：蔚杰 成斌[1] 贺学礼[1] 赵丽莉[1] YU Jie;CHENG Bin;HE Xueili;ZHAO Lili(The College of Life Sciences,Hebei University,Baoding 071002,China)

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北农业大学学报》2019年第1期57-64,共8页Journal of Hebei Agricultural University

基　　金：国家自然科学基金项目(31270460);河北省自然科学基金项目(C2014201060);河北省生物学强势特色学科项目

摘　　要：本研究于2015年在内蒙古正蓝旗青格勒图选取典型沙化梁地,设置坡底、坡中和坡顶3个样地,按0～10cm、10～20cm、20～30cm、30～40cm、40～50cm等5个土层采集羊柴(Hedysarum laeve Maxim)根围土壤样品。通过磷脂脂肪酸(PLFA)技术和Sherlock微生物鉴定系统,研究了不同样地土壤微生物群落结构以及土壤因子的生态功能。结果表明:(1)不同样地土壤微生物PLFA具有较高的多样性,在3个样地分别检测到30、31和26种磷脂脂肪酸(PLFA),构成土壤微生物群落的主要磷脂脂肪酸有15∶0anteiso、18∶1ω7c、18∶1ω9c、17∶0iso、17∶1ω7c10-methyl、18∶2ω6c、20∶0 10-methyl、17∶0anteiso、18∶1ω7c10-methyl、17∶1ω8c、18∶3ω6c、17∶0 10-methyl、16∶1ω5c、19∶3ω6c和19∶0anteiso。(2)羊柴根围土壤微生物主要有AM真菌(AMFungi)、革兰氏阴性菌(Gram Negative)、真核生物(Eukaryote)、真菌(Fungi)、革兰氏阳性菌(Gram Positive)、厌氧菌(Anaerobe)和放线菌(Actinomycetes)。各类微生物的含量表现为坡底>坡中>坡顶。(3)在3个样地中,以15∶0iso、15∶0anteiso、16∶0iso、16∶1ω7c、16∶1ω5c、16∶0 10-methyl、17∶0iso、17∶0anteiso、17∶0cycloω7c、17∶1isoω9c、18∶1ω9c、18∶1ω7c和19∶0cycloω7c等具有较高的丰富度和优势度。(4)坡底、坡中和坡顶样地AM真菌分别占到真菌生物量68.7%、67.7%和68.4%,表明AM真菌是沙化梁地土壤微生物系统中真菌的主要组成部分。(5)真核生物和革兰氏阴性菌与土壤总氮和有机碳显著正相关;AM真菌、真菌、放线菌和革兰氏阳性菌与有机碳、pH和湿度显著正相关;厌氧菌与土壤湿度显著正相关;各类微生物均与土壤总磷和速效磷显著负相关。The experiment was conducted in Qinggele Tu,Zhenglan Qi of Inner Mongolia in2015.The bottom,middle and top of the slope were set as three sample plots,and soil layers of 0~10 cm,10~20 cm,20~30 cm,30~40 cm,and 40~50 cm were chosen to collect Hedysarumlaeve Maxim rhizosphere soil samples.Through the technology of phospholipid fatty acids(PLFA)and the Sherlock microbiological identification system,the soil microbial community structure was analyzed in three different slopes of Qinggele Tu,Zhenglan Qi of Inner Mongolia.The results show that:(1)The PLFA diversity of microorganism is high in different soil samples.30,31 and 26 phospholipids(PLFA)are tested in three sample spots respectively,and the main phospholipid fatty acid in microbial community of Hedysarum laeve Maxim rhizosphere soil are 15∶0 anteiso,18∶1ω7 c,18∶1ω9 c,17∶0 iso,17∶1ω7 c10-methyl,18∶2ω6 c,20∶0 10-methyl,17∶0 anteiso,18∶1ω7 c10-methyl,17∶1ω8 c,18∶3ω6 c,17∶0 10-methyl,16∶1ω5 c,19∶3ω6 cand 19∶0 anteiso.(2)The microbes in Hedysarum laeve Maxim rhizosphere soil are mainly AM Fungi,gram negative bacteria,eukaryotes,fungi,gram positive bacteria,anaerobic bacteria and actinomycetes.The content of the microbes in different soil layers are:the bottom of the slope >the middle of the slope >the top of the slope.(3)In the three sample plots,15∶0 iso,15∶0 anteiso,16∶0 iso,16∶1ω7 c,16∶1ω5 c,16∶0 10-methyl,17∶0 iso,17∶0 anteiso,17∶0 cycloω7 c,17∶1 isoω9 c,18∶1ω9 c,18∶1ω7 cand 19∶0 cycloω7 chave high abundance and do minance.(4)AM fungi accounts for 68.7%,67.7% and 68.4% respectively of the total fungi in the bottom,middle and top of the slope soil,which indicated that AM fungi is an important part of the foungi in soil microorganism system in arid region of Inner Mongolia.(5)Eukaryotes and gram negative bacteria are significantly positively correlated with soil total nitrogen and organic carbon.AM fungi,fungi,actinomycetes and gram positive bacteria are significantly positively correlated wit

关 键 词：土壤微生物群落 磷脂脂肪酸(PLFA) 空间分布 羊柴 内蒙古沙化梁地 

分 类 号：S154.3[农业科学—土壤学]